Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors.
Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors.
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进化的酿酒酵母菌株的表型表征和比较转录组学具有提高木质纤维素衍生抑制剂的耐受性。
DOI：
10.1186/s13068-016-0614-y
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发表时间：
2016
影响因子：
6.3
通讯作者：
Doran-Peterson J
中科院分区：
文献类型：
--
作者：
Thompson OA;Hawkins GM;Gorsich SW;Doran-Peterson J
木质纤维素生物质继续被研究作为生物乙醇生产的可行来源。然而，预处理过程产生抑制性化合物，其损害微生物如酿酒酵母的生长和发酵性能。松木在获得工业相关的乙醇滴度方面特别具有挑战性。工业S。酿酒酵母菌在预处理的松树生物质中进行定向进化和适应，得到的菌株GHP 1和GHP 4在相关抑制化合物存在下在预处理的松树生物质上表现出改善的生长和发酵能力。应用比较转录组学方法来鉴定和表征进化菌株的表型稳定性差异。进化的菌株与预处理的松树表现出不同的发酵能力，这似乎受到预培养期间添加或不添加13种抑制化合物的影响。GHP 4的性能是一致的，与培养条件无关，而GHP 1的性能取决于与抑制剂的培养。比较转录组学揭示了52个可能与同时对多种抑制剂的应激反应相关的基因。荧光显微镜显示，改善细胞的完整性与线粒体表现出的抑制剂的破坏性影响的父母相比，这两个菌株。已经发现了多种潜在的新的遗传靶点，通过对我们进化的菌株的表征来理解胁迫耐受性。这项研究专门研究了多种抑制剂的协同作用，确定的目标将指导未来的研究，以补救抑制剂的影响，并进一步开发用于多种工业应用的强大酵母菌株。本文的在线版本（doi：10.1186/s13068-016-0614-y）包含补充材料，可供授权用户使用。
Lignocellulosic biomass continues to be investigated as a viable source for bioethanol production. However, the pretreatment process generates inhibitory compounds that impair the growth and fermentation performance of microorganisms such as Saccharomyces cerevisiae. Pinewood specifically has been shown to be challenging in obtaining industrially relevant ethanol titers. An industrial S. cerevisiae strain was subjected to directed evolution and adaptation in pretreated pine biomass and resultant strains, GHP1 and GHP4, exhibited improved growth and fermentative ability on pretreated pine in the presence of related inhibitory compounds. A comparative transcriptomic approach was applied to identify and characterize differences in phenotypic stability of evolved strains. Evolved strains displayed different fermentative capabilities with pretreated pine that appear to be influenced by the addition or absence of 13 inhibitory compounds during pre-culturing. GHP4 performance was consistent independent of culturing conditions, while GHP1 performance was dependent on culturing with inhibitors. Comparative transcriptomics revealed 52 genes potentially associated with stress responses to multiple inhibitors simultaneously. Fluorescence microscopy revealed improved cellular integrity of both strains with mitochondria exhibiting resistance to the damaging effects of inhibitors in contrast to the parent. Multiple potentially novel genetic targets have been discovered for understanding stress tolerance through the characterization of our evolved strains. This study specifically examines the synergistic effects of multiple inhibitors and identified targets will guide future studies in remediating effects of inhibitors and further development of robust yeast strains for multiple industrial applications. The online version of this article (doi:10.1186/s13068-016-0614-y) contains supplementary material, which is available to authorized users.
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影响因子：
6.3
作者：
Chen Y;Sheng J;Jiang T;Stevens J;Feng X;Wei N
通讯作者：
Wei N
影响因子：
6.3
作者：
Allen SA;Clark W;McCaffery JM;Cai Z;Lanctot A;Slininger PJ;Liu ZL;Gorsich SW
通讯作者：
Gorsich SW
影响因子：
6.3
作者：
Endo A;Nakamura T;Ando A;Tokuyasu K;Shima J
通讯作者：
Shima J
DOI：
10.1006/bbrc.1998.9864
发表时间：
1998-12-30
影响因子：
3.1
作者：
Grant, CM;Perrone, G;Dawes, IW
通讯作者：
Dawes, IW
影响因子：
17.3
作者：
HEIPIEPER, HJ;WEBER, FJ;DEBONT, JAM
通讯作者：
DEBONT, JAM