Development of anti-Crimean-Congo hemorrhagic fever virus Gc and NP-specific ELISA for detection of antibodies in domestic animal sera.
Development of anti-Crimean-Congo hemorrhagic fever virus Gc and NP-specific ELISA for detection of antibodies in domestic animal sera.
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DOI：
10.3389/fvets.2022.913046
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
克里米亚-刚果出血热(CCHF)是一种优先出现的疾病。由CCHF病毒(CCHFV)引起的CCHF可导致人类出血热，严重病例往往会造成致命后果。CCHFV维持在扁虱-脊椎动物-扁虱循环中，其中包括家畜。感染CCHFV的家畜没有表现出疾病的临床症状，血清中抗体的存在可以提供它们接触病毒的证据。目前的血清学检测是针对CCHFV的一种抗原或整个病毒抗原。在这里，我们介绍了两种用于检测血清中针对两种不同CCHFV抗原的特异性的ELISA：糖蛋白GC(CCHFV GC)和核蛋白(CCHFV NP)。我们证明，这两种方法都能检测到CCHFV流行区绵羊体内抗CCHFV GC特异性和抗CCHFV NP特异性的抗体，为研究家畜自然感染CCHFV诱导的免疫应答的异质性提供了新的视角。
Crimean-Congo hemorrhagic fever (CCHF) is a priority emerging disease. CCHF, caused by the CCHF virus (CCHFV), can lead to hemorrhagic fever in humans with severe cases often having fatal outcomes. CCHFV is maintained within a tick-vertebrate-tick cycle, which includes domestic animals. Domestic animals infected with CCHFV do not show clinical signs of the disease and the presence of antibodies in the serum can provide evidence of their exposure to the virus. Current serological tests are specific to either one CCHFV antigen or the whole virus antigen. Here, we present the development of two in-house ELISAs for the detection of serum IgG that is specific for two different CCHFV antigens: glycoprotein Gc (CCHFV Gc) and nucleoprotein (CCHFV NP). We demonstrate that these two assays were able to detect anti-CCHFV Gc-specific and anti-CCHFV NP-specific IgG in sheep from endemic CCHFV areas with high specificity, providing new insight into the heterogeneity of the immune response induced by natural infection with CCHFV in domestic animals.
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影响因子：
5.2
作者：
Shrivastava, Neha;Shrivastava, Ambuj;Dash, Paban Kumar
通讯作者：
Dash, Paban Kumar
DOI：
10.3390/v13030372
发表时间：
2021-02-26
期刊：
Viruses
影响因子：
--
作者：
Hartlaub J;von Arnim F;Fast C;Mirazimi A;Keller M;Groschup MH
通讯作者：
Groschup MH
影响因子：
7.8
作者：
Utrilla-Trigo S;Jiménez-Cabello L;Alonso-Ravelo R;Calvo-Pinilla E;Marín-López A;Moreno S;Lorenzo G;Benavides J;Gilbert S;Nogales A;Ortego J
通讯作者：
Ortego J
影响因子：
3.1
作者：
Dowall, S. D.;Richards, K. S.;Hewson, R.
通讯作者：
Hewson, R.
影响因子：
3.8
作者：
Mertens, Marc;Vatansever, Zati;Groschup, Martin H.
通讯作者：
Groschup, Martin H.