Update in Diagnostics of Toscana Virus Infection in a Hyperendemic Region (Southern Spain).

Update in Diagnostics of Toscana Virus Infection in a Hyperendemic Region (Southern Spain).
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DOI:
10.3390/v13081438
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发表时间:
2021-07-23
期刊:
Viruses
影响因子:
--
通讯作者:
Pérez-Ruiz M
Pérez-Ruiz M
中科院分区:
其他
文献类型:
--
作者:
Sanbonmatsu-Gámez S;Pedrosa-Corral I;Navarro-Marí JM;Pérez-Ruiz M

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白蛉热托斯卡纳病毒(TOSV,白蛉病毒属,白蛉病毒科)在地中海国家流行。在西班牙,TOSV毒株的系统发育研究表明,一种不同于意大利的基因型正在流行。本更新报告了1988年至2020年在安达卢西亚通过病毒培养、血清学和/或RT-PCR检测到的107例TOSV神经系统感染病例。大多数病例位于高流行区格拉纳达省。由于很少有实验室将该病毒纳入其研究组合,因此TOSV神经系统感染可能未得到充分诊断。这项工作提出了一种可靠的自动化方法,经验证可用于检测涉及急性脑膜炎和脑炎的主要病毒,包括虫媒病毒TOSV和西尼罗河病毒。该试验解决了对不同病毒的多个分子平台的需求,从而缩短了这些综合征获得结果的时间,这些综合征需要快速有效的诊断方法。
The sandfly fever Toscana virus (TOSV, genus Phlebovirus, family Phenuiviridae) is endemic in Mediterranean countries. In Spain, phylogenetic studies of TOSV strains demonstrated that a genotype, different from the Italian, was circulating. This update reports 107 cases of TOSV neurological infection detected in Andalusia from 1988 to 2020, by viral culture, serology and/or RT-PCR. Most cases were located in Granada province, a hyperendemic region. TOSV neurological infection may be underdiagnosed since few laboratories include this virus in their portfolio. This work presents a reliable automated method, validated for the detection of the main viruses involved in acute meningitis and encephalitis, including the arboviruses TOSV and West Nile virus. This assay solves the need for multiple molecular platforms for different viruses and thus, improves the time to results for these syndromes, which require a rapid and efficient diagnostic approach.
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