Vascularization strategies in tissue engineering approaches for soft tissue repair.
Vascularization strategies in tissue engineering approaches for soft tissue repair.
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DOI：
10.1002/term.3225
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发表时间：
2021-09
影响因子：
3.3
通讯作者：
Tayebi L
中科院分区：
文献类型：
--
作者：
Masson-Meyers DS;Tayebi L
组织修复过程中血管化不足通常与不良的临床结果相关。这是一个值得关注的问题，尤其是当患者患有严重损伤时，缺损的大小限制了血管分布，甚至是必须在特殊条件下治疗的小缺损，例如阻碍血管分布的放射治疗（与肿瘤切除相关）后。事实上，血管化不良是组织工程方法在软组织修复临床应用的主要障碍之一。一个关键问题是，植入后血管化不足导致移植物整合不足，可能导致移植物失败。此外，由于缺氧和营养供应不足，血管化不良会损害植入支架/移植材料深层的细胞的活力。在本文中，我们旨在回顾组织工程技术中用于修复软组织的血管化策略。为此，我们首先简要概述软组织生理伤口愈合过程中的主要事件。然后，我们讨论如何通过组织工程实现组织修复，以及支架材料的选择、培养条件和血管化技术的考虑因素。接下来，我们强调血管化的重要性，以及软组织等效物的预血管化的策略和方法，特别是基于细胞的预血管化。最后，我们总结了组织工程软组织结构血管化过程中常用的体外方法。
Insufficient vascularization during tissue repair is often associated with poor clinical outcomes. This is a concern especially when patients have critical-sized injuries, where the size of the defect restricts vascularity, or even in small defects that have to be treated under special conditions, such as after radiation therapy (relevant to tumor resection) that hinders vascularity. In fact, poor vascularization is one of the major obstacles for clinical application of tissue engineering methods in soft tissue repair. As a key issue, lack of graft integration, caused by inadequate vascularization after implantation, can lead to graft failure. Moreover, poor vascularization compromises the viability of cells seeded in deep portions of scaffolds/graft materials, due to hypoxia and insufficient nutrient supply. In this article we aim to review vascularization strategies employed in tissue engineering techniques to repair soft tissues. For this purpose, we start by providing a brief overview of the main events during the physiological wound healing process in soft tissues. Then, we discuss how tissue repair can be achieved through tissue engineering, and considerations with regards to the choice of scaffold materials, culture conditions, and vascularization techniques. Next, we highlight the importance of vascularization, along with strategies and methods of prevascularization of soft tissue equivalents, particularly cell-based prevascularization. Lastly, we present a summary of commonly used in vitro methods during the vascularization of tissue-engineered soft tissue constructs.
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影响因子：
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