Mating increases neuronal tyrosine hydroxylase expression and selectively gates transmission of male chemosensory information in female mice.
Mating increases neuronal tyrosine hydroxylase expression and selectively gates transmission of male chemosensory information in female mice.
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DOI：
10.1371/journal.pone.0069943
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Brennan PA
中科院分区：
文献类型：
--
作者：
Matthews GA;Patel R;Walsh A;Davies O;Martínez-Ricós J;Brennan PA
暴露于来自陌生雄性的化学感受信号可以终止最近交配的雌性小鼠的妊娠。在主嗅球中的酪氨酸羟化酶阳性神经元的数量已被发现增加交配后，并已牵连在防止男性诱导的妊娠阻滞在植入后期间。相反，植入前妊娠阻滞是由犁鼻系统介导的，并且被认为是通过在副嗅球水平选择性抑制配偶的妊娠阻滞化学信号来预防的。本研究的目的是首先确定犁鼻途径的水平，在该水平上选择性抑制配偶的妊娠阻断化学信号发生。第二，研究交配后犁鼻系统酪氨酸羟化酶阳性神经元是否增加，这可能在预防植入前妊娠阻滞中发挥作用。免疫组织化学染色显示，交配诱导增加酪氨酸羟化酶阳性神经元在弓状下丘脑的BALB/c女性，并抑制c-Fos在这些神经元中的表达，交配男性的化学信号。这种选择性抑制c-Fos反应交配雄性化学信号是不明显的早期水平的妊娠阻断神经通路的副嗅球或皮质杏仁核。免疫组织化学染色显示，交配后的BALB/c雌性小鼠的副嗅球中的酪氨酸羟化酶阳性神经元的数量增加。然而，增加多巴胺介导的抑制在副嗅球是不太可能占预防怀孕块交配男性，酪氨酸羟化酶的表达没有增加女性的C57 BL/6株，这表明正常的配偶识别。这些研究结果揭示了一个协会的交配增加多巴胺能调制在妊娠阻断途径，并支持这一假设，配偶识别防止妊娠阻断抑制激活弓状多巴胺释放。
Exposure to chemosensory signals from unfamiliar males can terminate pregnancy in recently mated female mice. The number of tyrosine hydroxylase-positive neurons in the main olfactory bulb has been found to increase following mating and has been implicated in preventing male-induced pregnancy block during the post-implantation period. In contrast, pre-implantation pregnancy block is mediated by the vomeronasal system, and is thought to be prevented by selective inhibition of the mate’s pregnancy blocking chemosignals, at the level of the accessory olfactory bulb. The objectives of this study were firstly to identify the level of the vomeronasal pathway at which selective inhibition of the mate’s pregnancy blocking chemosignals occurs. Secondly, to determine whether a post-mating increase in tyrosine hydroxylase-positive neurons is observed in the vomeronasal system, which could play a role in preventing pre-implantation pregnancy block. Immunohistochemical staining revealed that mating induced an increase in tyrosine-hydroxylase positive neurons in the arcuate hypothalamus of BALB/c females, and suppressed c-Fos expression in these neurons in response to mating male chemosignals. This selective suppression of c-Fos response to mating male chemosignals was not apparent at earlier levels of the pregnancy-blocking neural pathway in the accessory olfactory bulb or corticomedial amygdala. Immunohistochemical staining revealed an increase in the number of tyrosine hydroxylase-positive neurons in the accessory olfactory bulb of BALB/c female mice following mating. However, increased dopamine-mediated inhibition in the accessory olfactory bulb is unlikely to account for the prevention of pregnancy block to the mating male, as tyrosine hydroxylase expression did not increase in females of the C57BL/6 strain, which show normal mate recognition. These findings reveal an association of mating with increased dopaminergic modulation in the pregnancy block pathway and support the hypothesis that mate recognition prevents pregnancy block by suppressing the activation of arcuate dopamine release.
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