Quantitative assessment of articular cartilage morphology via EPIC-microCT.
Quantitative assessment of articular cartilage morphology via EPIC-microCT.
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DOI：
10.1016/j.joca.2008.07.015
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发表时间：
2009-03
影响因子：
7
通讯作者：
Guldberg RE
中科院分区：
文献类型：
--
作者：
Xie L;Lin AS;Levenston ME;Guldberg RE
本研究的目的是验证EPIC-μCT在大鼠模型中非破坏性评估软骨形态的能力。确定适当的造影剂（Hexabrix）浓度和平衡孵育时间，以便从对比增强μCT扫描中对股骨关节软骨进行可重复分割。通过对6个股骨进行三次扫描来评估生殖能力，并将测得的关节软骨厚度与针探针测试和组织学测定的厚度进行独立比较。然后应用经验证的技术来量化4、8和16周龄（每组n=5）雄性Wistar大鼠之间关节软骨形态的年龄相关差异。在大鼠模型中，40%Hexabrix/60%PBS溶液孵育30分钟对于将软骨从下层骨组织和其他软组织中分割出来是最佳的。软骨体积、厚度和表面积的变异系数低（1.7-2.5%）表明具有高重现性。厚度的EPIC-μCT评价显示出强线性关系，并且与针探（r2=0.95，斜率=0.81，p<0.01，平均差异11±22μm，n=43）和组织学（r2=0.99，斜率=0.97，p<0.01，平均差异12±10μm，n=30）具有良好的一致性。随着年龄的增长，腕骨的体积和厚度显着下降，而表面积显着增加。EPIC-μCT成像能够在小动物模型中以高精度和准确度非破坏性地评估三维关节软骨形态。
The objective of the present study was to validate the ability of EPIC-μCT to nondestructively assess cartilage morphology in the rat model. An appropriate contrast agent (Hexabrix) concentration and incubation time for equilibration were determined for reproducible segmentation of femoral articular cartilage from contrast-enhanced μCT scans. Reproducibility was evaluated by triplicate scans of six femora, and the measured articular cartilage thickness was independently compared to thickness determined from needle probe testing and histology. The validated technique was then applied to quantify age-related differences in articular cartilage morphology between 4, 8, and 16-week old (n=5 each) male Wistar rats. A 40% Hexabrix/60% PBS solution with 30 minute incubation was optimal for segmenting cartilage from the underlying bone tissue and other soft tissues in the rat model. High reproducibility was indicated by the low coefficient of variation (1.7-2.5%) in cartilage volume, thickness and surface area. EPIC-μCT evaluation of thickness showed a strong linear relationship and good agreement with both needle probing (r2=0.95, slope=0.81, p<0.01, mean difference 11±22μm, n=43) and histology (r2=0.99, slope=0.97, p<0.01, mean difference 12±10μm, n=30). Cartilage volume and thickness significantly decreased with age while surface area significantly increased. EPIC-μCT imaging has the ability to nondestructively evaluate three-dimensional articular cartilage morphology with high precision and accuracy in a small animal model.
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影响因子：
2.4
作者：
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