CAP1 is overexpressed in human epithelial ovarian cancer and promotes cell proliferation.
CAP1 is overexpressed in human epithelial ovarian cancer and promotes cell proliferation.
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CAP1在人类上皮卵巢癌中过表达，并促进细胞增殖。
DOI：
10.3892/ijmm.2015.2089
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发表时间：
2015-04
影响因子：
5.4
通讯作者：
Zhong J
中科院分区：
文献类型：
--
作者：
Hua M;Yan S;Deng Y;Xi Q;Liu R;Yang S;Liu J;Tang C;Wang Y;Zhong J
腺苷酸环化酶相关蛋白1 （CAP1）在酵母中调节肌动蛋白丝和Ras/cAMP通路，并在细胞运动和某些类型癌症的发展中发挥作用。在本研究中，我们研究了CAP1基因在人上皮性卵巢癌（EOC）中的表达。对EOC组织样本进行Western blot和免疫组化分析，结果显示CAP1的表达随着EOC分级的增加而增加。而在正常卵巢组织样本中，几乎检测不到CAP1的表达。经Pearson χ2检验，CAP1表达与组织分级及Ki-67表达相关。Kaplan-Meier分析显示，在EOC患者中，较高的CAP1表达与较差的预后相关。在HO-8910 EOC细胞的体外实验中，使用siRNA敲低CAP1的表达。采用细胞计数试剂盒-8和流式细胞术进行细胞周期分析和细胞增殖试验，检测HO-8910细胞的增殖情况。结果表明，CAP1表达的缺失抑制了细胞周期的进展。这些发现提示CAP1的高表达参与了EOC的发病机制，下调肿瘤细胞中CAP1的表达可能是治疗EOC患者的治疗靶点。
Adenylate cyclase-associated protein 1 (CAP1) regulates both actin filaments and the Ras/cAMP pathway in yeast, and has been found play a role in cell motility and in the development of certain types of cancer. In the present study, we investigated CAP1 gene expression in human epithelial ovarian cancer (EOC). Western blot analysis and immunohistochemistry were performed using EOC tissue samples and the results revealed that CAP1 expression increased with the increasing grade of EOC. In the normal ovarian tissue samples however, CAP1 expression was barely detected. Using Pearson’s χ2 test, it was demonstrated that CAP1 expression was associated with the histological grade and Ki-67 expression. Kaplan-Meier analysis revealed that a higher CAP1 expression in patients with EOC was associated with a poorer prognosis. In in vitro experiments using HO-8910 EOC cells, the expression of CAP1 was knocked down using siRNA. The proliferation of the HO-8910 cells was then determined by cell cycle analysis and cell proliferation assay using the cell counting kit-8 and flow cytometry. The results revealed that the loss of CAP1 expression inhibited cell cycle progression. These findings suggest that a high expression of CAP1 is involved in the pathogenesis of EOC, and that the downregulation of CAP1 in tumor cells may be a therapeutic target for the treatment of patients with EOC.
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影响因子：
2.4
作者：
Li, Mei;Yang, Xiaojing;Zhang, Jianguo
通讯作者：
Zhang, Jianguo
影响因子：
5.3
作者：
MATVIW, H;YU, G;YOUNG, D
通讯作者：
YOUNG, D
影响因子：
3.5
作者：
SWISTON, J;HUBBERSTEY, A;YOUNG, D
通讯作者：
YOUNG, D
影响因子：
64.5
作者：
VOJTEK, A;HAARER, B;WIGLER, M
通讯作者：
WIGLER, M
影响因子：
5
作者：
Yamazaki, Ken;Takamura, Masaaki;Sakamoto, Michiie
通讯作者：
Sakamoto, Michiie