Low-dose mifepristone increased angiogenesis in a manner involving AQP1
Low-dose mifepristone increased angiogenesis in a manner involving AQP1
复制标题
低剂量米非司酮通过涉及 AQP1 的方式增加血管生成
DOI：
10.1007/s00404-018-4989-9
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发表时间：
2018-12
影响因子：
2.6
通讯作者：
Huang Lili
中科院分区：
文献类型：
--
作者：
Zhou Feng;Qian Zhida;Huang Lili
目的探讨水通道蛋白-1 （AQP1）介导的米非司酮诱导血管生成的分子机制，提高对小剂量米非司酮作为抗着床避孕药的认识。方法以人脐静脉内皮细胞（HUVECs）为实验对象，探讨不同浓度米非司酮（0、65、200 nmol/L）对其血管生成活性的影响。用不同浓度的米非司酮治疗45只处于“着床窗口期”的怀孕小鼠。采用甲基噻唑四氮唑（MTT）法检测HUVECs的增殖。采用免疫组化方法检测子宫内膜微血管密度（MVD）和AQP1的表达。结果MVD和AQP1表达明显高于对照组。200 nmol/L的米非司酮显著影响HUVECs在12 h培养过程中的增殖，aqp1特异性siRNA预处理显著抑制了米非司酮增强的细胞增殖。结论慢剂量米非司酮以参与AQP1的方式促进血管生成。这为低剂量米非司酮血管生成作用的分子机制提供了新的见解。
PurposeTo investigate the molecular mechanisms governing aquaporin-1 (AQP1)-mediated, mifepristone-induced angiogenesis and improve the understanding of low-dose mifepristone serving as an anti-implantation contraceptive drug.MethodsHuman umbilical vein endothelial cells (HUVECs) were used to explore the effects of different concentrations of mifepristone (0, 65, and 200 nmol/L) on the activity of angiogenesis. Forty-five pregnant mice during the “window of implantation” were treated with different concentrations of mifepristone. HUVECs’ proliferation was examined using a methyl thiazolyl tetrazolium (MTT) assay. The microvessel density (MVD) and the expression of AQP1 in endometrium were determined with immunohistochemical methods.ResultsThe MVD and the expression of AQP1 were significantly higher than controls. Mifepristone at 200 nmol/L significantly affected HUVECs’ proliferation during culture over 12 h, and pretreatment with AQP1-specific siRNA significantly inhibited the mifepristone-enhanced cell proliferation.ConclusionsLow-dose mifepristone increased angiogenesis in a manner involving AQP1. This affords a new insight into the molecular mechanism underpinning the angiogenic effects of low-dose mifepristone.
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DOI：
--
发表时间：
1991
期刊：
Allergie und Immunologie
影响因子：
--
作者：
H. Weichert;I. Blechschmidt;S. Schröder;H. Ambrosius
通讯作者：
H. Weichert;I. Blechschmidt;S. Schröder;H. Ambrosius
DOI：
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发表时间：
1976-10
期刊：
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影响因子：
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158.5
作者：
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2005-08
影响因子：
2.1
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绝经前女性子宫内膜血管AQP1和子宫内膜上皮AQP2表达降低与无排卵子宫出血相关
DOI：
10.1097/gme.0b013e31816086ef
发表时间：
2008-07-01
影响因子：
2.7
作者：
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通讯作者：
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