Spatial and Temporal Variations in Pigment and Species Compositions of Snow Algae on Mt. Tateyama in Toyama Prefecture, Japan.
Spatial and Temporal Variations in Pigment and Species Compositions of Snow Algae on Mt. Tateyama in Toyama Prefecture, Japan.
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DOI：
10.3389/fpls.2021.689119
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发表时间：
2021
影响因子：
5.6
通讯作者：
Takeuchi N
中科院分区：
文献类型：
--
作者：
Nakashima T;Uetake J;Segawa T;Procházková L;Tsushima A;Takeuchi N
雪藻是一种光合作用微生物，生活在高山和极地融化的雪面上。对山上采集的彩色雪进行了色素和种类组成的分析。在2015年和2016年的融化季节，Tateyama在日本。对彩雪中提取的色素进行了高效液相色谱分析，结果表明其组成在研究区内各不相同，可分为A型(虾青素-单酯为主)、B型(中等含量)、C型(富含初级类胡萝卜素和游离虾青素)和D型(富含初级类胡萝卜素和虾青素双酯)。A型和B型在研究区域最常见，而C型和D型只出现在特定的地点。对18S核糖体RNA(18S RRNA)基因的分析揭示了雪藻的6个主要扩增序列变异(ASV)，分别属于血藻、氯单胞菌和氯单胞菌。A型和B型藻类ASV的相对丰度均以血藻为主(>；48%)，说明这两种藻类虾青素丰度的差异是由藻细胞中色素的产生引起的。C型和D型藻类群落结构与A型和B型藻类群落结构不同，表明初级类胡萝卜素和虾青素双酯来源于这些类型中的某些藻种。因此，富含虾青素的血根藻主要诱导该高寒地区广泛出现的红雪，但部分以绿单胞菌或氯单胞藻为主，产生不同的色素组成。
Snow algae are photosynthetic microbes that inhabit the melting snow surface in alpine and polar regions. We analyzed the pigment and species composition of colored snow collected on Mt. Tateyama in Japan during the melting seasons of 2015 and 2016. High-performance liquid chromatographic analyses of the pigments extracted from the colored snow showed that their composition varied within the study area and were classified into four types: Type A (astaxanthin-monoester dominant), Type B (medium astaxanthin-monoester content), Type C (abundant primary carotenoids and free-astaxanthin), and Type D (abundant primary carotenoids and astaxanthin diesters). Types A and B were most commonly observed in the study area, whereas Types C and D appeared only at specific sites. Analysis of the 18S ribosomal RNA (18S rRNA) gene revealed six major amplicon sequence variants (ASVs) of snow algae, belonging to the Sanguina, Chloromonas, and Chlainomonas groups. The relative abundance of the algal ASVs showed that Sanguina was dominant (>48%) in both Types A and B, suggesting that the difference in astaxanthin abundance between the two types was caused by the production of pigments in the algal cells. The algal community structures of Types C and D differed from those of Types A and B, indicating that the primary carotenoids and astaxanthin diesters were derived from certain algal species in these types. Therefore, astaxanthin-rich Sanguina algae mostly induced the red snow that appeared widely in this alpine area; however, they were partially dominated by Chloromonas or Chlainomonas algae, causing different pigment compositions.
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影响因子：
2.6
作者：
Brown SP;Tucker AE
通讯作者：
Tucker AE
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.6
作者：
Fujii, Masanori;Takano, Yoshinori;Kojima, Hisaya;Hoshino, Tamotsu;Tanaka, Ryouichi;Fukui, Manabu
通讯作者：
Fukui, Manabu
影响因子：
1
作者：
Muramoto, Kyohei;Kato, Syou;Nozaki, Hisayoshi
通讯作者：
Nozaki, Hisayoshi
影响因子：
2.3
作者：
Brown, Shawn P.;Ungerer, Mark C.;Jumpponen, Ari
通讯作者：
Jumpponen, Ari