Effect of endothelium mimicking self-assembled nanomatrices on cell adhesion and spreading of human endothelial cells and smooth muscle cells.
Effect of endothelium mimicking self-assembled nanomatrices on cell adhesion and spreading of human endothelial cells and smooth muscle cells.
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DOI：
10.1016/j.nano.2009.09.004
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发表时间：
2010-04
影响因子：
5.4
通讯作者：
Jun, Ho-Wook
中科院分区：
文献类型：
--
作者：
Andukuri, Adinarayana;Minor, Will P.;Kushwaha, Meenakshi;Anderson, Joel M.;Jun, Ho-Wook
本研究的目的是开发独特的天然内皮模拟纳米基质，并评估其对人脐静脉内皮细胞（HUVECs）和主动脉平滑肌细胞（AoSMCs）粘附和铺展的影响。这些纳米基质是通过溶剂蒸发技术由肽两亲物（PA）的自组装开发的。开发了三种PA，一种含有Tyr-Ile-Gly-Ser-Arg（YIGSR）配体，第二种含有Val-Ala-Pro-Gly（VAPG）配体，第三种不含细胞粘附配体。分别通过PicoGreen-DNA测定和Live/Dead测定评价细胞粘附和铺展。我们的结果表明，与其他PA相比，PA-YIGSR显著增强HUVEC粘附（26704±2708）、铺展（84 ±8%）和增殖（50±2%）。与PA-S相比，PA-VAPG和PA-YIGSR显示出显著更大的AoSMC粘附。与其他PA相比，PA-VAPG还显示出显著更大的AoSMC扩散（63 ±11%）。此外，与I型胶原（对照）相比，所有PA均显示血小板粘附显著降低。这些发现将促进新型血管移植物、心脏瓣膜和基于细胞的心血管疾病治疗的发展。
The goal of this study is to develop unique native endothelium mimicking nanomatrices and evaluate their effects on adhesion and spreading of human umbilical vein endothelial cells (HUVECs) and aortic smooth muscle cells (AoSMCs). These nanomatrices were developed by self-assembly of peptide amphiphiles (PAs) through a solvent evaporation technique. Three PAs, one containing the Tyr-Ile-Gly-Ser-Arg (YIGSR) ligand, second containing the Val-Ala-Pro-Gly (VAPG) ligand, and a third without cell adhesive ligands were developed. Cell adhesion and spreading were evaluated by a PicoGreen-DNA assay and Live/Dead assay respectively. Our results show that PA-YIGSR significantly enhances HUVEC adhesion (26704±2708) spreading (84 ±8%), and proliferation (50±2%) when compared to other PAs. PA-VAPG and PA-YIGSR showed significantly greater AoSMC adhesion when compared to PA-S. PA-VAPG also showed significantly greater spreading of AoSMCs (63 ±11%) when compared with other PAs. Also, all the PAs showed significantly reduced platelet adhesion when compared with collagen I (control). These findings would facilitate the development of novel vascular grafts, heart valves, and cell based therapies for cardiovascular diseases.
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DOI：
10.1002/jbm.820250209
发表时间：
1991-02-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
MASSIA, SP;HUBBELL, JA
通讯作者：
HUBBELL, JA
DOI：
10.1163/156856204322752246
发表时间：
2004-01-01
影响因子：
3.6
作者：
Jun, HW;West, J
通讯作者：
West, J
DOI：
10.1002/jbm.b.30135
发表时间：
2005-01-15
影响因子：
3.4
作者：
Jun, HW;West, JL
通讯作者：
West, JL
影响因子：
14
作者：
Fittkau, MH;Zilla, P;Davies, N
通讯作者：
Davies, N
影响因子：
3.7
作者：
Mitchell, SL;Niklason, LE
通讯作者：
Niklason, LE