Tapetum and middle layer control male fertility in Actinidia deliciosa.
Tapetum and middle layer control male fertility in Actinidia deliciosa.
复制标题
DOI：
10.1093/aob/mct173
复制
发表时间：
2013-10
期刊：
影响因子：
4.2
通讯作者：
Altamura MM
中科院分区：
文献类型：
--
作者：
Falasca G;D'Angeli S;Biasi R;Fattorini L;Matteucci M;Canini A;Altamura MM
雌雄异株是许多具有经济价值的作物的特征，包括猕猴桃（美味猕猴桃）。猕猴桃雄性不育发生在小孢子期。雄性不育花和雄性可育花的小孢子和花粉的细胞壁分别具有不同的葡萄糖和半乳糖水平。在许多植物中，花粉形成涉及绒毡层的正常功能和退化时间，绒毡层提供的钙和碳水化合物对雄性育性至关重要。本研究的目的是确定猕猴桃花药壁是否控制雄性育性，为小孢子提供钙和碳水化合物。利用光学显微镜、荧光显微镜、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL法）和透射电镜结合电子能谱等技术，对雄性可育和雄性不育花药的花药壁和小孢子中发生的事件进行了研究。雄性不育的可能性，花药组织功能障碍方面的钙/葡萄糖/半乳糖提供给小孢子也进行了研究，在体外花药培养。绒毡层和中间层均表现出分泌活性，均发生程序性细胞死亡，但不育花药程序性细胞死亡晚于可育花药。钙主要分布在花药中层细胞壁、绒毡层细胞壁、小孢子和花粉外壁，在不育花药壁组织和死亡小孢子中含量较高。一个特定的供应葡萄糖和钙诱导正常花粉形成在体外培养的花药的雄性不育基因型。结果表明，猕猴桃雄性不育是由花药壁组织，特别是中层细胞的细胞程序性死亡延迟引起的分泌活动延长而引起的。离体培养结果支持孢子体控制猕猴桃雄性育性，并为克服雌雄异株和优化猕猴桃生产开辟了道路。
Dioecism characterizes many crop species of economic value, including kiwifruit (Actinidia deliciosa). Kiwifruit male sterility occurs at the microspore stage. The cell walls of the microspores and the pollen of the male-sterile and male-fertile flowers, respectively, differ in glucose and galactose levels. In numerous plants, pollen formation involves normal functioning and degeneration timing of the tapetum, with calcium and carbohydrates provided by the tapetum essential for male fertility. The aim of this study was to determine whether the anther wall controls male fertility in kiwifruit, providing calcium and carbohydrates to the microspores. The events occurring in the anther wall and microspores of male-fertile and male-sterile anthers were investigated by analyses of light microscopy, epifluorescence, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and transmission electron microscopy coupled with electron spectroscopy. The possibility that male sterility was related to anther tissue malfunctioning with regard to calcium/glucose/galactose provision to the microspores was also investigated by in vitro anther culture. Both tapetum and the middle layer showed secretory activity and both degenerated by programmed cell death (PCD), but PCD was later in male-sterile than in male-fertile anthers. Calcium accumulated in cell walls of the middle layer and tapetum and in the exine of microspores and pollen, reaching higher levels in anther wall tissues and dead microspores of male-sterile anthers. A specific supply of glucose and calcium induced normal pollen formation in in vitro-cultured anthers of the male-sterile genotype. The results show that male sterility in kiwifruit is induced by anther wall tissues through prolonged secretory activity caused by a delay in PCD, in the middle layer in particular. In vitro culture results support the sporophytic control of male fertility in kiwifruit and open the way to applications to overcome dioecism and optimize kiwifruit production.
登录
查看更多内容
影响因子：
6.9
作者：
Engelke T;Hirsche J;Roitsch T
通讯作者：
Roitsch T
雌雄异株猕猴桃（猕猴桃）中富含基因的连锁图谱揭示了假定的 X/Y 性别决定染色体。
影响因子：
4.4
作者：
Fraser, Lena G.;Tsang, Gianna K.;Datson, Paul M.;De Silva, H. Nihal;Harvey, Catherine F.;Gill, Geoffrey P.;Crowhurst, Ross N.;McNeilage, Mark A.
通讯作者：
McNeilage, Mark A.
影响因子：
6.9
作者：
BHADULA, SK;SAWHNEY, VK
通讯作者：
SAWHNEY, VK
影响因子：
4.3
作者：
Castro, Antonio J.;Clement, Christophe
通讯作者：
Clement, Christophe
影响因子：
6.5
作者：
Falasca, G.;Franceschetti, M.;Biasi, R.
通讯作者：
Biasi, R.