Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries.
Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries.
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DOI：
10.1177/0883911512458050
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发表时间：
2012-11
影响因子：
1.7
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Fan Y;Wen ZT;Liao S;Lallier T;Hagan JL;Twomley JT;Zhang JF;Sun Z;Xu X
最近，利用重组全长淀粉原蛋白与氟化物结合，在形成致密排列的搪瓷样结构方面显示出有希望的结果。本研究利用含钙、磷酸盐和氟化物的淀粉原蛋白（rP172）释放水凝胶，对早期牙釉质龋模型进行再矿化效果的研究。将水凝胶应用于人工拔牙的人第三磨牙的人工龋齿，并在不同的模型中测试其再矿化效果：人工唾液存在下的静态凝胶再矿化，pH为5.4的醋酸缓冲液和pH为7.3的凝胶再矿化，以及在连续流动的定深膜发酵罐中培养的多物种口腔生物膜。从水凝胶中释放出淀粉原蛋白后，再矿化牙釉质表面显微硬度明显升高。矿化水凝胶对牙周膜细胞无细胞毒性作用。
Recently, the use of recombinant full-length amelogenin protein in combination with fluoride has shown promising results in the formation of densely packed enamel-like structures. In this study, amelogenin (rP172)-releasing hydrogels containing calcium, phosphate, and fluoride were investigated for remineralization efficacy using in vitro early enamel caries models. The hydrogels were applied to artificial caries lesions on extracted human third molars, and the remineralization efficacy was tested in different models: static gel remineralization in the presence of artificial saliva, pH cyclic treatment at pH 5.4 acetic buffer and pH 7.3 gel remineralization, and treatment with multispecies oral biofilms grown in a continuous flowing constant-depth film fermenter. The surface microhardness of remineralized enamel increased significantly when amelogenin was released from hydrogel. No cytotoxicity was observed when periodontal ligament cells were cultured with the mineralized hydrogels.
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影响因子：
2.7
作者：
Chang, Shou-Yi;Chung, Ren-Jei;Hsu, Hung-Bin
通讯作者：
Hsu, Hung-Bin
影响因子：
4
作者：
Cheng, Zhen-Jiang;Wang, Xiu-Mei;Yan, Jian-Xin
通讯作者：
Yan, Jian-Xin
影响因子：
1.9
作者：
Oshiro, Maki;Yamaguchi, Kanako;Miyazaki, Masashi
通讯作者：
Miyazaki, Masashi
影响因子：
2.1
作者：
Bitoun JP;Nguyen AH;Fan Y;Burne RA;Wen ZT
通讯作者：
Wen ZT
影响因子：
3
作者：
Beniash, Elia;Metzler, Rebecca A.;Lam, Raymond S. K.;Gilbert, P. U. P. A.
通讯作者：
Gilbert, P. U. P. A.