A physicochemical double-cross-linked gelatin hydrogel with enhanced antibacterial and anti-inflammatory capabilities for improving wound healing.
A physicochemical double-cross-linked gelatin hydrogel with enhanced antibacterial and anti-inflammatory capabilities for improving wound healing.
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DOI：
10.1186/s12951-022-01634-z
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发表时间：
2022-09-24
影响因子：
10.2
通讯作者：
Shen, Xuanri
中科院分区：
文献类型：
--
作者：
Lu, Yapeng;Zhao, Meihui;Peng, Ye;He, Sizhe;Zhu, Xiaopeng;Hu, Chao;Xia, Guanghua;Zuo, Tao;Zhang, Xueying;Yun, Yonghuan;Zhang, Weimin;Shen, Xuanri
皮肤组织在保护身体免受伤害和细菌感染方面至关重要。细菌定植引起的创面感染是阻碍创面愈合的主要因素之一。大量细菌定植引起的伤口感染可使创面进入持续的炎症阶段，从而延缓创面愈合。水凝胶创面敷料是由天然和人工合成的聚合物组成，它能吸收组织液，改善创面局部微环境，促进创面愈合。但在水凝胶制备过程中，制备工艺复杂，生物功效差，限制了水凝胶伤口敷料在复杂伤口环境中的应用。因此，利用天然聚合物开发和制备工艺简单、物性好、生物效应好的水凝胶敷料就显得尤为重要。在本研究中，通过将三价铁（Fe3+）和2,3,4-三羟基苯甲醛（THA）混合形成配合物（THA& fe），然后与罗非鱼皮明胶（Tsg）进行简单的希夫碱反应，制备了明胶基（Tsg-THA&Fe）水凝胶。通过控制络合物的数量来调节凝胶的凝胶时间和流变性能。配位键（邻苯三酚- fe3 +）和席夫碱键的动态交联使水凝胶具有良好的自愈性和可注射性。体外实验证实该水凝胶具有良好的生物相容性和生物降解性，并具有粘附、止血、抗菌等性能。通过对大鼠皮肤创伤模型的治疗，研究了Tsg-THA&Fe水凝胶的可行性。结果表明，与Comfeel®Plus透明敷料相比，Tsg-THA&Fe水凝胶可以明显减少微生物数量，防止细菌定植，减轻炎症，加速伤口愈合。Tsg-THA&Fe水凝胶在皮肤组织的局部分布没有引起器官毒性。综上所述，Tsg-THA&Fe水凝胶的制备工艺简单，具有优异的物理性能和生物功效。可有效缓解炎症，控制创面微生物定植，可作为多功能敷料促进创面愈合。在线版本包含补充材料，可在10.1186/s12951-022-01634-z获得。
Skin tissue is vital in protecting the body from injuries and bacterial infections. Wound infection caused by bacterial colonization is one of the main factors hindering wound healing. Wound infection caused by colonization of a large number of bacteria can cause the wound to enter a continuous stage of inflammation, which delays wound healing. Hydrogel wound dressing is composed of natural and synthetic polymers, which can absorb tissue fluid, improve the local microenvironment of wound, and promote wound healing. However, in the preparation process of hydrogel, the complex preparation process and poor biological efficacy limit the application of hydrogel wound dressing in complex wound environment. Therefore, it is particularly important to develop and prepare hydrogel dressings with simple technology, good physical properties and biological effects by using natural polymers. In this study, a gelatin-based (Tsg-THA&Fe) hydrogel was created by mixing trivalent iron (Fe3+) and 2,3,4-trihydroxybenzaldehyde (THA) to form a complex (THA&Fe), followed by a simple Schiff base reaction with tilapia skin gelatin (Tsg). The gel time and rheological properties of the hydrogels were adjusted by controlling the number of complexes. The dynamic cross-linking of the coordination bonds (o-phthalmictriol-Fe3+) and Schiff base bonds allows hydrogels to have good self-healing and injectable properties. In vitro experiments confirmed that the hydrogel had good biocompatibility and biodegradability as well as adhesion, hemostasis, and antibacterial properties. The feasibility of Tsg-THA&Fe hydrogel was studied by treating rat skin trauma model. The results showed that compared with Comfeel® Plus Transparent dressing, the Tsg-THA&Fe hydrogel could obvious reduce the number of microorganisms, prevent bacterial colonization, reduce inflammation and accelerate wound healing. Local distribution of the Tsg-THA&Fe hydrogel in the skin tissue did not cause organ toxicity. In summary, the preparation process of Tsg-THA&Fe hydrogel is simple, with excellent performance in physical properties and biological efficacy. It can effectively relieve inflammation and control the colonization of wound microbes, and can be used as a multi-functional dressing to improve wound healing. The online version contains supplementary material available at 10.1186/s12951-022-01634-z.
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影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M
DOI：
10.1016/j.msec.2019.01.135
发表时间：
2019-06-01
影响因子：
7.9
作者：
Acevedo, Cristian A.;Olguin, Yusser;Ortiz, Rina
通讯作者：
Ortiz, Rina
仿生多功能混合水凝胶促进伤口愈合
影响因子：
19
作者：
Chen, Guopu;Yu, Yunru;Zhao, Yuanjin
通讯作者：
Zhao, Yuanjin
影响因子：
13.6
作者：
Blacklow, S. O.;Li, J.;Mooney, D. J.
通讯作者：
Mooney, D. J.
用于软骨组织工程的可注射酶交联羧甲基化支链淀粉/硫酸软骨素水凝胶
影响因子：
4.6
作者：
Chen F;Yu S;Liu B;Ni Y;Yu C;Su Y;Zhu X;Yu X;Zhou Y;Yan D
通讯作者：
Yan D