The importance of morphological identification of African anopheline mosquitoes (Diptera: Culicidae) for malaria control programmes.
The importance of morphological identification of African anopheline mosquitoes (Diptera: Culicidae) for malaria control programmes.
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非洲瞬间蚊子（双翅目：Culicidae）对疟疾控制程序的形态鉴定的重要性。
DOI：
10.1186/s12936-018-2189-5
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发表时间：
2018-01-22
期刊：
影响因子：
3
通讯作者：
Coetzee M
中科院分区：
文献类型：
--
作者：
Erlank E;Koekemoer LL;Coetzee M
正确查明病媒是实施有效控制方案的第一步。传统上，对于疟疾控制，这是基于在不同蚊子物种之间的成虫和幼虫中观察到的形态差异。然而，物种复合体的发现意味着需要遗传工具来分离兄弟物种，今天有标准的分子技术用于识别蚊子的两个主要疟疾媒介群。假设物种诊断DNA聚合酶链反应（PCR）检测具有高度的物种特异性，进行了实验，以研究如果将非载体物种随机纳入分子检测中会发生什么。非洲热带按蚊亚科的形态检索表被用来提供先验的识别。然后对所有蚊子标本进行冈比亚按蚊复合群和不死按蚊群成员的标准PCR检测。150只蚊子属于11个形态的物种进行了处理。3种按蚊（Anopheles pretoriensis、Anopheles rufipes和Anopheles rhodesiensis）扩增了该基因。funestus group和4个种（An. pretoriensis、隐翅虫An. rufipes、Anopheles listeri和Anopheles squamosus）扩增的成员。冈比亚情结。在进行聚合酶链反应分析之前对蚊子进行形态学鉴定不仅可以节省实验室的时间和金钱，而且还可以确保疟疾病媒控制方案收到的数据有助于确定主要病媒。
The correct identification of disease vectors is the first step towards implementing an effective control programme. Traditionally, for malaria control, this was based on the morphological differences observed in the adults and larvae between different mosquito species. However, the discovery of species complexes meant that genetic tools were needed to separate the sibling species and today there are standard molecular techniques that are used to identify the two major malaria vector groups of mosquitoes. On the assumption that species-diagnostic DNA polymerase chain reaction (PCR) assays are highly species-specific, experiments were conducted to investigate what would happen if non-vector species were randomly included in the molecular assays. Morphological keys for the Afrotropical Anophelinae were used to provide the a priori identifications. All mosquito specimens were then subjected to the standard PCR assays for members of the Anopheles gambiae complex and Anopheles funestus group. One hundred and fifty mosquitoes belonging to 11 morphological species were processed. Three species (Anopheles pretoriensis, Anopheles rufipes and Anopheles rhodesiensis) amplified members of the An. funestus group and four species (An. pretoriensis, An. rufipes, Anopheles listeri and Anopheles squamosus) amplified members of the An. gambiae complex. Morphological identification of mosquitoes prior to PCR assays not only saves time and money in the laboratory, but also ensures that data received by malaria vector control programmes are useful for targeting the major vectors.
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影响因子：
4.6
作者：
Lobo NF;St Laurent B;Sikaala CH;Hamainza B;Chanda J;Chinula D;Krishnankutty SM;Mueller JD;Deason NA;Hoang QT;Boldt HL;Thumloup J;Stevenson J;Seyoum A;Collins FH
通讯作者：
Collins FH
DOI：
10.4269/ajtmh.2003.69.200
发表时间：
2003-08-01
影响因子：
3.3
作者：
Cohuet, A;Simard, F;Fontenille, D
通讯作者：
Fontenille, D
影响因子：
1.9
作者：
MAHON, RJ;GREEN, CA;HUNT, RH
通讯作者：
HUNT, RH
影响因子：
--
作者：
Kyalo, David;Amratia, Punam;Snow, Robert W
通讯作者：
Snow, Robert W
影响因子：
2.1
作者：
Awono-Ambene, HP;Kengne, P;Fontenille, D
通讯作者：
Fontenille, D