Multiplexed specific label-free detection of NCI-H358 lung cancer cell line lysates with silicon based photonic crystal microcavity biosensors.
Multiplexed specific label-free detection of NCI-H358 lung cancer cell line lysates with silicon based photonic crystal microcavity biosensors.
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DOI：
10.1016/j.bios.2012.11.012
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发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Chen RT
中科院分区：
文献类型：
--
作者：
Chakravarty S;Lai WC;Zou Y;Drabkin HA;Gemmill RM;Simon GR;Chin SH;Chen RT
我们通过实验证明了绝缘体上硅 (SOI) 中的无标记光子晶体 (PC) 微腔生物传感器可检测微量样品中的上皮间质转化 (EMT) 转录因子 ZEB1。证明了对 NCI-H358 肺癌细胞裂解物中的 ZEB1 进行多重特异性检测，其估计浓度低至每微升 2 个细胞。采用与 W1 光子晶体波导耦合的 L13 光子晶体微腔，其中共振在生物环境磷酸盐缓冲盐水 (PBS) 中显示出高 Q 值。当传感器表面衍生有特定抗体时，来自复杂全细胞裂解物的相应抗原的结合会在光子晶体微腔附近产生折射率的变化，从而导致光子晶体微腔共振模式的共振波长的变化。共振波长的变化揭示了抗原的存在。传感器腔体的表面积约为 11 μm2。多重传感器允许在同一时刻同时检测与来自同一生物样品的特定固定化抗体的许多结合相互作用。使用夹心测定法证明了特异性，该夹心测定法进一步增强了低浓度下的检测灵敏度。该设备代表了在硅芯片平台上对癌细胞进行无标记、高通量、多重检测的概念验证，具有特异性和敏感性。
We experimentally demonstrate label-free photonic crystal (PC) microcavity biosensors in silicon-on-insulator (SOI) to detect the epithelial-mesenchymal transition (EMT) transcription factor, ZEB1, in minute volumes of sample. Multiplexed specific detection of ZEB1 in lysates from NCI-H358 lung cancer cells down to an estimated concentration of 2 cells per micro-liter is demonstrated. L13 photonic crystal microcavities, coupled to W1 photonic crystal waveguides, are employed in which resonances show high Q in the bio-ambient phosphate buffered saline (PBS). When the sensor surface is derivatized with a specific antibody, the binding of the corresponding antigen from a complex whole-cell lysate generates a change in refractive index in the vicinity of the photonic crystal microcavity, leading to a change in the resonance wavelength of the resonance modes of the photonic crystal microcavity. The shift in the resonance wavelength reveals the presence of the antigen. The sensor cavity has a surface area of ~11 μm2. Multiplexed sensors permit simultaneous detection of many binding interactions with specific immobilized antibodies from the same bio-sample at the same instant of time. Specificity was demonstrated using a sandwich assay which further amplifies the detection sensitivity at low concentrations. The device represents a proof-of-concept demonstration of label-free, high throughput, multiplexed detection of cancer cells with specificity and sensitivity on a silicon chip platform.
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DOI：
10.1073/pnas.1734137100
发表时间：
2003-09-02
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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