Genetic analysis of the "head top shape" quality trait of Chinese cabbage and its association with rosette leaf variation.
Genetic analysis of the "head top shape" quality trait of Chinese cabbage and its association with rosette leaf variation.
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大白菜“头顶形”品质性状遗传分析及其与莲座叶变异的关联
DOI：
10.1038/s41438-021-00541-y
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发表时间：
2021-05-01
影响因子：
8.7
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Sun X;Gao Y;Lu Y;Zhang X;Luo S;Li X;Liu M;Feng D;Gu A;Chen X;Xuan S;Wang Y;Shen S;Bonnema G;Zhao J
大白菜的农业品质和消费品质由其形状决定。形状由结球叶片的折叠情况决定，这也决定了球顶形状（HTS）。重叠的球顶形状，即结球叶片向内弯曲并在顶部重叠，是消费者喜爱的形状。为了解球顶形状的遗传调控机制，我们利用小白菜和大白菜杂交构建了一个大型的F2分离群体，其表型从非结球到结球不等，结球叶片有向外弯曲或向内弯曲重叠的情况。球顶形状与株高、外叶/莲座叶长度以及叶柄长度相关。构建了一个高密度遗传图谱。数量性状位点（QTL）分析鉴定出22个与叶球相关性状的QTL，其中包括5个球顶形状QTL。利用混合分组分析法（BSA）对球顶形状QTL进行了验证，并基于有信息的单核苷酸多态性鉴定了候选基因。有趣的是，球顶形状QTL与株高、外叶/莲座叶和叶柄长度的QTL共定位，这与观察到的表型相关性一致。QTL分析和混合分组分析法相结合，为大白菜球顶形状的分子标记辅助育种以及该性状遗传调控的进一步研究方向奠定了基础。
The agricultural and consumer quality of Chinese cabbage is determined by its shape. The shape is defined by the folding of the heading leaves, which defines the head top shape (HTS). The overlapping HTS, in which the heading leaves curve inward and overlap at the top, is the shape preferred by consumers. To understand the genetic regulation of HTS, we generated a large segregating F2 population from a cross between pak choi and Chinese cabbage, with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves. HTS was correlated with plant height, outer/rosette leaf length, and petiole length. A high-density genetic map was constructed. Quantitative trait locus (QTL) analysis resulted in the identification of 22 QTLs for leafy head-related traits, which included five HTS QTLs. Bulked segregant analysis (BSA) was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms. Interestingly, the HTS QTLs colocalized with QTLs for plant height, outer/rosette leaf, and petiole length, consistent with the observed phenotypic correlations. Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait.
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影响因子：
7.2
作者：
Horiguchi, Gorou;Fujikura, Ushio;Tsukaya, Hirokazu
通讯作者：
Tsukaya, Hirokazu
DOI：
10.1016/j.bbrc.2017.06.123
发表时间：
2017-08-26
影响因子：
3.1
作者：
Kim, Man -Sun;Hong, Seongmin;Lim, Yong Pyo
通讯作者：
Lim, Yong Pyo
栽培花生 (Arachis hypergaea L.) 荚果大小和重量主要数量性状基因座在 A05 染色体上 3.7 cM 间隔的共定位
影响因子：
4.4
作者：
Luo H;Ren X;Li Z;Xu Z;Li X;Huang L;Zhou X;Chen Y;Chen W;Lei Y;Liao B;Pandey MK;Varshney RK;Guo B;Jiang X;Liu F;Jiang H
通讯作者：
Jiang H
影响因子：
3.7
作者：
Elshire RJ;Glaubitz JC;Sun Q;Poland JA;Kawamoto K;Buckler ES;Mitchell SE
通讯作者：
Mitchell SE
影响因子：
44.1
作者：
He, YK;Xue, WX;Liu, PL
通讯作者：
Liu, PL