Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice.
Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice.
复制标题
微电极阵列记录窦房结放电频率以识别小鼠内在心脏起搏缺陷。
DOI：
10.3791/62735
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发表时间：
2021-07-05
期刊：
影响因子：
--
通讯作者：
Glasscock E
中科院分区：
文献类型：
--
作者：
Kumar P;Si M;Paulhus K;Glasscock E
位于右心房的窦房结（SAN）包含心脏的起搏细胞，该区域的功能障碍可导致心动过速或心动过缓。心脏起搏缺陷的可靠识别需要通过在很大程度上防止自主神经系统的影响来测量固有心率，自主神经系统可以掩盖心率缺陷。分析固有心脏起搏器功能的传统方法包括药物诱导的自主神经阻滞以测量体内心率，隔离心脏记录以测量固有心率，以及窦房条或窦房起搏细胞的单细胞膜片钳记录以测量自发动作电位放电率。然而，这些更传统的技术可能在技术上具有挑战性并且难以执行。在这里，我们提出了一种新的方法来测量固有的心脏放电率进行微电极阵列（MEA）记录的整体安装窦房结准备从小鼠。MEA由多个以网格状图案排列的微电极组成，用于记录体外胞外场电位。本文所述的方法具有比先前用于记录固有心率的方法相对更快、更简单和更精确的组合优点，同时还允许容易的药理学询问。
The sinoatrial node (SAN), located in the right atrium, contains the pacemaker cells of the heart, and dysfunction of this region can cause tachycardia or bradycardia. Reliable identification of cardiac pacemaking defects requires the measurement of intrinsic heart rates by largely preventing the influence of the autonomic nervous system, which can mask rate deficits. Traditional methods to analyze intrinsic cardiac pacemaker function include drug-induced autonomic blockade to measure in vivo heart rates, isolated heart recordings to measure intrinsic heart rates, and sinoatrial strip or single-cell patch-clamp recordings of sinoatrial pacemaker cells to measure spontaneous action potential firing rates. However, these more traditional techniques can be technically challenging and difficult to perform. Here, we present a new methodology to measure intrinsic cardiac firing rate by performing microelectrode array (MEA) recordings of whole-mount sinoatrial node preparations from mice. MEAs are composed of multiple microelectrodes arranged in a grid-like pattern for recording in vitro extracellular field potentials. The method described herein has the combined advantage of being relatively faster, simpler, and more precise than previous approaches for recording intrinsic heart rates, while also allowing easy pharmacological interrogation.
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