FigA, a Putative Homolog of Low-Affinity Calcium System Member Fig1 in Saccharomyces cerevisiae, Is Involved in Growth and Asexual and Sexual Development in Aspergillus nidulans
FigA, a Putative Homolog of Low-Affinity Calcium System Member Fig1 in Saccharomyces cerevisiae, Is Involved in Growth and Asexual and Sexual Development in Aspergillus nidulans
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FigA 是酿酒酵母中低亲和力钙系统成员 Fig1 的假定同源物，参与构巢曲霉的生长以及无性和有性发育
DOI：
10.1128/ec.00257-13
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Ling Lu
中科院分区：
文献类型：
--
作者：
Natalia Carbó;Peiying Chen;Pablo S. Aguilar;Ling Lu
摘要钙离子介导的信号通路在真核生物中广泛存在，参与多种生物过程的调控。在酿酒酵母中，已经鉴定出至少两种不同的钙摄取系统：高亲和力钙流入系统（HACS）和低亲和力钙流入系统（拉克）。与HACS相比，真菌中的拉克并不为人所知。在本研究中，FigA是S.酿酒酵母，功能特点是在丝状真菌构巢曲霉。无花果A的损失导致延迟菌丝生长和分生孢子生产急剧减少。最重要的是，FigA是同宗配合（自花受精）过程中必不可少的;此外，FigA是异宗配合（异交）在缺乏HACS midA。有趣的是，在figA缺失突变体中，加入胞外Ca 2+挽救了菌丝生长缺陷，但不能恢复无性繁殖和有性繁殖。此外，定量PCR结果显示，figA缺失急剧降低了brlA和nsdD的表达，这两个基因分别是无性和有性发育过程中的关键调控因子。此外，在FigA的C端标记绿色荧光蛋白（GFP）（FigA：：GFP）表明，FigA定位于成熟菌丝细胞的隔膜中心，分生孢子柄的囊泡与后壁之间，以及后壁与瓶梗的连接处。因此，我们的研究结果表明，FigA除了是A. nidulans在菌丝生长、无性和有性发育过程中可能发挥多种未知的作用。
ABSTRACT Calcium-mediated signaling pathways are widely employed in eukaryotes and are implicated in the regulation of diverse biological processes. In Saccharomyces cerevisiae, at least two different calcium uptake systems have been identified: the high-affinity calcium influx system (HACS) and the low-affinity calcium influx system (LACS). Compared to the HACS, the LACS in fungi is not well known. In this study, FigA, a homolog of the LACS member Fig1 from S. cerevisiae, was functionally characterized in the filamentous fungus Aspergillus nidulans. Loss of figA resulted in retardant hyphal growth and a sharp reduction of conidial production. Most importantly, FigA is essential for the homothallic mating (self-fertilization) process; further, FigA is required for heterothallic mating (outcrossing) in the absence of HACS midA. Interestingly, in a figA deletion mutant, adding extracellular Ca2+ rescued the hyphal growth defects but could not restore asexual and sexual reproduction. Furthermore, quantitative PCR results revealed that figA deletion sharply decreased the expression of brlA and nsdD, which are known as key regulators during asexual and sexual development, respectively. In addition, green fluorescent protein (GFP) tagging at the C terminus of FigA (FigA::GFP) showed that FigA localized to the center of the septum in mature hyphal cells, to the location between vesicles and metulae, and between the junctions of metulae and phialides in conidiophores. Thus, our findings suggest that FigA, apart from being a member of a calcium uptake system in A. nidulans, may play multiple unexplored roles during hyphal growth and asexual and sexual development.
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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