Ciprofloxacin is a potential topoisomerase II inhibitor for the treatment of NSCLC.
Ciprofloxacin is a potential topoisomerase II inhibitor for the treatment of NSCLC.
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DOI：
10.3892/ijo.2012.1653
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发表时间：
2012-12
影响因子：
5.2
通讯作者：
Drewa T
中科院分区：
文献类型：
--
作者：
Kloskowski T;Gurtowska N;Olkowska J;Nowak JM;Adamowicz J;Tworkiewicz J;Dębski R;Grzanka A;Drewa T
肺癌是最常见的肿瘤之一，其治疗仍然效率低下。在我们以前的工作中，我们证明了环丙沙星对五种癌细胞系有不同的影响。在此，我们的目的是比较环丙沙星对治疗后代表不同反应的细胞系的生物学效应，因此选择A549作为敏感模型，C6和B16作为高度耐药模型。分析了三种不同的细胞系（A549、B16和C6）。用实时细胞分析仪（RTCA）-DP系统分析连续细胞生长的表征。使用免疫荧光证实细胞骨架的变化。使用流式细胞术分析细胞周期。环丙沙星仅对A549细胞株具有细胞生长抑制作用。在其他测试细胞系的情况下，未观察到细胞生长抑制作用。细胞骨架分析证实了RTCA-DP获得的结果。A549细胞被抑制在G2/M期，提示与拓扑异构酶II抑制有关的机制。环丙沙星的生物学效应支持这一假设，即由于其能够抑制拓扑异构酶II的特性，该药物可以作为肺癌的辅助治疗。
Lung cancer is one of the most common tumors and its treatment is still inefficient. In our previous work we proved that ciprofloxacin has a different influence on five cancer cell lines. Here, we aimed to compare the biological effect of ciprofloxacin on cell lines representing different responses after treatment, thus A549 was chosen as a sensitive model, C6 and B16 as highly resistant. Three different cell lines were analyzed (A549, B16 and C6). The characterization of continuous cell growth was analyzed with the Real-Time Cell Analyzer (RTCA)-DP system. Cytoskeletal changes were demonstrated using immunofluorescence. The cell cycle was analyzed using flow cytometry. Ciprofloxacin was cytostatic only against the A549 cell line. In the case of other tested cell lines a cytostatic effect was not observed. Cytoskeletal analysis confirms the results obtained with RTCA-DP. A549 cells were inhibited in the G2/M phase suggesting a mechanism related to topoisomerase II inhibition. The biological effects of ciprofloxacin support the hypothesis that this drug can serve as an adjuvant treatment for lung cancer, due to its properties enabling topoisomerase II inhibition.
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影响因子：
2.1
作者：
Chono, Sumio;Tanino, Tomoharu;Morimoto, Kazuhiro
通讯作者：
Morimoto, Kazuhiro
影响因子：
9.7
作者：
Benes, Petr;Knopfova, Lucia;Smarda, Jan
通讯作者：
Smarda, Jan
DOI：
10.1007/978-1-61779-108-6_6
发表时间：
2011-01-01
期刊：
MAMMALIAN CELL VIABILITY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Ke, Ning;Wang, Xiaobo;Abassi, Yama A.
通讯作者：
Abassi, Yama A.
影响因子：
4.5
作者：
Gurbay, Aylin;Gonthier, Brigitte;Hincal, Filiz
通讯作者：
Hincal, Filiz
影响因子：
2.5
作者：
Haraguchi, Shuji;Hioki, Masafumi;Shimizu, Kazuo
通讯作者：
Shimizu, Kazuo