Porphyromonas gingivalis accelerates inflammatory atherosclerosis in the innominate artery of ApoE deficient mice.
Porphyromonas gingivalis accelerates inflammatory atherosclerosis in the innominate artery of ApoE deficient mice.
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DOI：
10.1016/j.atherosclerosis.2010.12.009
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发表时间：
2011-03
期刊：
影响因子：
5.3
通讯作者：
Genco, Caroline A.
中科院分区：
文献类型：
--
作者：
Hayashi, Chie;Viereck, Jason;Hua, Ning;Phinikaridou, Alkystis;Madrigal, Andres G.;Gibson, Frank C., III;Hamilton, James A.;Genco, Caroline A.
人类研究支持口腔病原体牙龈卟啉单胞菌在炎症性动脉粥样硬化发展中的作用。本研究的目的是确定牙龈卟啉卟啉菌感染是否会加速小鼠无名动脉的炎症和动脉粥样硬化，据报道，小鼠无名动脉表现出许多人类动脉粥样硬化疾病的特征，包括斑块破裂。载脂蛋白e缺陷（ApoE−/−）小鼠口服感染牙龈卟啉单胞菌，并使用磁共振成像（MRI）监测活小鼠动脉粥样硬化的进展。与未感染的小鼠相比，感染牙龈卟啉卟啉菌的小鼠在无名动脉中显示出具有统计学意义的动脉粥样硬化斑块增加。偏光显微镜和免疫组织化学显示，与未感染小鼠相比，感染小鼠的无名动脉中脂质、巨噬细胞和T细胞增加。在病原体暴露前用热杀灭牙龈假单胞菌免疫可以防止斑块、总胆固醇酯和胆固醇一水晶体、巨噬细胞和T细胞的增加。这些研究首次通过病原体暴露后的体内MRI分析证明了炎症斑块在无名动脉中的积累进展，并通过免疫证明了对无名动脉斑块进展的保护。
Studies in humans support a role for the oral pathogen Porphyromonas gingivalis in the development of inflammatory atherosclerosis. The goal of this study was to determine if P. gingivalis infection accelerates inflammation and atherosclerosis in the innominate artery of mice, an artery which has been reported to exhibit many features of human atherosclerotic disease, including plaque rupture. Apolipoprotein E-deficient (ApoE−/−) mice were orally infected with P. gingivalis, and Magnetic Resonance Imaging (MRI) was used to monitor the progression of atherosclerosis in live mice. P. gingivalis infected mice exhibited a statistically significant increase in atherosclerotic plaque in the innominate artery as compared to uninfected mice. Polarized light microscopy and immunohistochemistry revealed that the innominate arteries of infected mice had increased lipids, macrophages and T cells as compared to uninfected mice. Increases in plaque, total cholesterol esters and cholesterol monohydrate crystals, macrophages, and T cells were prevented by immunization with heat-killed P. gingivalis prior to pathogen exposure. These are the first studies to demonstrate progression of inflammatory plaque accumulation in the innominate arteries by in-vivo MRI analysis following pathogen exposure, and to document protection from plaque progression in the innominate artery via immunization.
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影响因子：
37.8
作者：
Desvarieux, M;Demmer, RT;Papapanou, PN
通讯作者：
Papapanou, PN
DOI：
10.1161/01.atv.0000078603.90302.4a
发表时间：
2003-07-01
影响因子：
8.7
作者：
Amar, S;Gokce, N;Vita, JA
通讯作者：
Vita, JA
影响因子：
3.1
作者：
Gibson, FC;Genco, CA
通讯作者：
Genco, CA
影响因子：
4.3
作者：
Haraszthy, VI;Zambon, JJ;Genco, RJ
通讯作者：
Genco, RJ
影响因子：
5.3
作者：
Andreeva, ER;Pugach, IM;Orekhov, AN
通讯作者：
Orekhov, AN