An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration.
An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration.
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DOI：
10.3389/fbioe.2020.559494
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发表时间：
2020
影响因子：
5.7
通讯作者：
El-Gendy R
中科院分区：
文献类型：
--
作者：
El-Sayed B;Davies RPW;El-Zehery RR;Ibrahim FM;Grawish ME;Kirkham J;El-Gendy R
牙周病是世界范围内最常见的疾病之一。它对口腔健康和个人的生活质量有着重要的影响。然而，牙周组织的最佳再生，使用目前的治疗，尚未实现。多肽自组装技术为纳米生物技术和再生医学领域提供了一个新的突破口。我们的目的是研究自组装肽（SAP; P11-4）对牙周再生的临床前模型的影响。在13只大鼠中，通过手术造成26个双侧上颌临界大小的牙周缺损。用P11-4水凝胶填充口腔一侧的缺损;对侧缺损未经处理（对照）。术后即刻（时间0）以及2周和4周后处死大鼠。对取回的上颌骨进行组织学、免疫组织化学和组织形态学评估。组织学分析的结果显示，与未处理的缺损相比，在两个时间点，用P11-4处理的缺损中牙周纤维的组织化更大。组织形态计量学显示，4周后，经治疗的缺损具有功能性牙周膜长度的显著增加和上皮向下生长的减少。在2周时，经处理的缺损显示出骨钙素和骨保护素表达的显著增加，如通过免疫组织化学判断的。此外，在治疗的缺损中显示了显著更高的骨保护素/RANKL比率。总之，结果表明，当SAP P11-4用于填充大鼠牙周缺损时，牙周组织的再生增强。这项研究的结果表明，SAP P11-4是一个有前途的新的候选牙周再生治疗。在临床使用前，需要进行进一步的研究以进行优化。
Periodontal disease is one of the most common diseases worldwide. It has a significant impact on oral health and subsequently the individual’s quality of life. However, optimal regeneration of periodontal tissues, using current treatments, has yet to be achieved. Peptide self-assembly has provided a step-change in nanobiotechnology and regenerative medicine fields. Our aim was to investigate the effects of a self-assembling peptide (SAP; P11-4) on periodontal regeneration in a preclinical model. Twenty-six bilateral maxillary critical-sized periodontal defects were created surgically in 13 rats. Defects on one side of the mouth were filled with P11-4 hydrogel; the contra-lateral defect was untreated (control). Rats were sacrificed immediately post-surgery (time 0) and after 2 and 4 weeks. Retrieved maxillae were processed for histological, immunohistochemical, and histomorphometric assessments. The results of histological analysis showed greater organization of periodontal fibers in defects treated with P11-4, at both time points, when compared to untreated defects. Histomorphometry showed that treated defects had both a significant increase in functional periodontal ligament length and a reduction in epithelial down growth after 4 weeks. At 2 weeks, treated defects showed a significant increase in expression of osteocalcin and osteoprotegerin as judged by immunohistochemistry. Also, a significantly higher osteoprotegerin/RANKL ratio was shown in treated defects. In conclusion, the results demonstrated enhanced regeneration of periodontal tissues when SAP P11-4 was used to fill periodontal defects in rats. The findings of this study suggest that SAP P11-4 is a promising novel candidate for periodontal regenerative therapy. Further investigations are required for optimization before clinical use.
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影响因子：
3.7
作者：
Peng X;Guo W;Ren T;Lou Z;Lu X;Zhang S;Lu Q;Sun Y
通讯作者：
Sun Y
DOI：
10.1002/jbm.b.31226
发表时间：
2009-05-01
影响因子：
3.4
作者：
Meng, Hui;Chen, Liyan;Zhao, Xiaojun
通讯作者：
Zhao, Xiaojun
DOI：
10.1073/pnas.97.12.6728
发表时间：
2000-06-06
影响因子：
11.1
作者：
Holmes, TC;de Lacalle, S;Zhang, SG
通讯作者：
Zhang, SG
影响因子：
3.4
作者：
Oortgiesen, Daniel A. W.;Meijer, Gert J.;Bronckers, Antonius L. J. J.;Walboomers, X. Frank;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
18.6
作者：
Petersen, Poul E.;Ogawa, Hiroshi
通讯作者：
Ogawa, Hiroshi