Protein profiling of human lung telocytes and microvascular endothelial cells using iTRAQ quantitative proteomics
Protein profiling of human lung telocytes and microvascular endothelial cells using iTRAQ quantitative proteomics
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使用 iTRAQ 定量蛋白质组学对人肺远程细胞和微血管内皮细胞进行蛋白质分析
DOI：
10.1111/jcmm.12350
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发表时间：
2014-06
影响因子：
5.3
通讯作者：
Wang XD
中科院分区：
文献类型：
--
作者：
Cretoiu SM;Popescu LM;Fang H;Wang XD
端细胞（TC）被描述为间隙空间的特定类型的细胞（www.telocytes.com）。其主要特征是端足极长，足节和足节交替。最近，我们进行了比较蛋白质组学分析的人肺TC与成纤维细胞，证明TC显然是一种独特的细胞类型。因此，本研究旨在通过比较肺TC与内皮细胞（EC）来加强这一观点，因为TC和EC对CD 34具有免疫阳性性。我们应用同量异位素标记相对和绝对定量（iTRAQ）结合自动化2-D nano-ESI LC-MS/MS分析从原代细胞培养物中的TC和EC提取的蛋白质。在细胞培养物中总共鉴定了1609种蛋白质。98种蛋白质（第5天）和82种蛋白质（第10天）被确信地定量（通过双样本t检验筛选，P < 0.05）为上调或下调（倍数变化>2）。我们发现，在TC中，在第5天有38个上调蛋白，在第10天有26个上调蛋白。利用Panther进行的生物信息学分析显示，与TC相关的38个蛋白质代表细胞功能，如细胞间通讯（通过囊泡介导的运输）和结构形态发生，主要是细胞骨架蛋白和氧化还原酶。此外，我们在EC中发现了60种上调的蛋白质，例如：细胞表面糖蛋白MUC 18（15.54倍）和血管性血友病因子（5.74倍）。还分析了第10天TC中的26种上调蛋白，并证实了相同的主要细胞功能，而56种下调蛋白再次证实了它们对EC的特异性。总之，我们在这里报告的第一个广泛的比较蛋白质从TC和EC使用定量蛋白质组学方法。我们的数据表明，TC与EC完全不同。蛋白质表达谱显示TC在细胞间通讯和细胞间信号传导中发挥特定作用。此外，它们可能抑制氧化应激和细胞衰老，并可能通过抑制细胞凋亡而具有促增殖作用。本研究中鉴定的蛋白质组需要进一步探索在肺部疾病发病机制中的作用。
Telocytes (TCs) are described as a particular type of cells of the interstitial space (www.telocytes.com). Their main characteristics are the very long telopodes with alternating podoms and podomers. Recently, we performed a comparative proteomic analysis of human lung TCs with fibroblasts, demonstrating that TCs are clearly a distinct cell type. Therefore, the present study aims to reinforce this idea by comparing lung TCs with endothelial cells (ECs), since TCs and ECs share immunopositivity for CD34. We applied isobaric tag for relative and absolute quantification (iTRAQ) combined with automated 2‐D nano‐ESI LC‐MS/MS to analyse proteins extracted from TCs and ECs in primary cell cultures. In total, 1609 proteins were identified in cell cultures. 98 proteins (the 5th day), and 82 proteins (10th day) were confidently quantified (screened by two‐sample t‐test, P < 0.05) as up‐ or down‐regulated (fold change >2). We found that in TCs there are 38 up‐regulated proteins at the 5th day and 26 up‐regulated proteins at the 10th day. Bioinformatics analysis using Panther revealed that the 38 proteins associated with TCs represented cellular functions such as intercellular communication (via vesicle mediated transport) and structure morphogenesis, being mainly cytoskeletal proteins and oxidoreductases. In addition, we found 60 up‐regulated proteins in ECs e.g.: cell surface glycoprotein MUC18 (15.54‐fold) and von Willebrand factor (5.74‐fold). The 26 up‐regulated proteins in TCs at 10th day, were also analysed and confirmed the same major cellular functions, while the 56 down‐regulated proteins confirmed again their specificity for ECs. In conclusion, we report here the first extensive comparison of proteins from TCs and ECs using a quantitative proteomics approach. Our data show that TCs are completely different from ECs. Protein expression profile showed that TCs play specific roles in intercellular communication and intercellular signalling. Moreover, they might inhibit the oxidative stress and cellular ageing and may have pro‐proliferative effects through the inhibition of apoptosis. The group of proteins identified in this study needs to be explored further for the role in pathogenesis of lung disease.
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影响因子：
5.3
作者：
Chen X;Zheng Y;Manole CG;Wang X;Wang Q
通讯作者：
Wang Q
影响因子：
--
作者：
Wang, Jian-Guang;Xu, Wei-Dong;Jiao, Bing-Hua
通讯作者：
Jiao, Bing-Hua
影响因子：
3.6
作者：
Hinescu, Mihail E.;Gherghiceanu, Mihaela;Suciu, Laura;Popescu, Laurentiu M.
通讯作者：
Popescu, Laurentiu M.
肝脏中的特洛细胞：电子显微镜和免疫荧光证据
影响因子：
5.3
作者：
Xiao J;Wang F;Liu Z;Yang C
通讯作者：
Yang C
影响因子：
5.3
作者：
Díaz-Flores L;Gutiérrez R;Sáez FJ;Díaz-Flores L Jr;Madrid JF
通讯作者：
Madrid JF