Microbial dysbiosis in colorectal cancer (CRC) patients.
Microbial dysbiosis in colorectal cancer (CRC) patients.
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DOI：
10.1371/journal.pone.0016393
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发表时间：
2011-01-27
期刊：
影响因子：
3.7
通讯作者：
Furet JP
中科院分区：
文献类型：
--
作者：
Sobhani I;Tap J;Roudot-Thoraval F;Roperch JP;Letulle S;Langella P;Corthier G;Tran Van Nhieu J;Furet JP
人体肠道微生物群的组成与健康状况有关。目的是分析正常和结肠癌患者的微生物群，以建立癌症相关的生态失调。在结肠镜检查前从179名患者中提取粪便细菌DNA：60名结肠直肠癌患者和119名正常结肠镜检查患者。对通过焦磷酸测序获得的12个粪便样品（6个正常和6个癌症）的细菌基因进行验证的主成分分析（PCA）测试。优势菌群和亚优势菌群（C. leptum，C.类球菌属、拟杆菌/普雷沃氏菌属、乳杆菌/明串珠菌/片球菌属、双歧杆菌属和E.大肠杆菌和普氏粪杆菌物种）的感染，并使用免疫组织化学表征肠粘膜中的特异性IL 17生产细胞。焦磷酸测序（最小序列200个核苷酸读数）显示，基于分类器软件的默认参数，所有序列的80%可以被分配到总共819个分类群。根据主成分分析，癌症个体的系统发育核心与正常个体不同，细菌的优势和亚优势家族有差异的趋势。因此，根据qPCR值，正常和癌症个体中的全细菌[log 10（细菌/g粪便）]相似[分别为11.88±0.35和11.80±0.56，（P = 0.16）]，而在所有优势和亚优势菌种中，只有拟杆菌/普雷沃氏菌的全细菌[log 10（细菌/g粪便）]在癌症个体中（-1.04 <$0.55）高于正常个体（-1.40 <$0.83）。 IL 17免疫反应阳性细胞在结肠癌患者的正常结肠黏膜中的表达明显高于结肠镜检查正常的结肠黏膜。这是第一个证明结肠癌患者微生物群组成变化的大型系列，可能影响粘膜免疫反应。这些数据为大规模筛查和病理生理学研究开辟了新的领域。
The composition of the human intestinal microbiota is linked to health status. The aim was to analyze the microbiota of normal and colon cancer patients in order to establish cancer-related dysbiosis. Stool bacterial DNA was extracted prior to colonoscopy from 179 patients: 60 with colorectal cancer, and 119 with normal colonoscopy. Bacterial genes obtained by pyrosequencing of 12 stool samples (6 Normal and 6 Cancer) were subjected to a validated Principal Component Analysis (PCA) test. The dominant and subdominant bacterial population (C. leptum, C. coccoides, Bacteroides/Prevotella, Lactobacillus/Leuconostoc/Pediococcus groups, Bifidobacterium genus, and E. coli, and Faecalibacterium prausnitzii species) were quantified in all individuals using qPCR and specific IL17 producer cells in the intestinal mucosa were characterized using immunohistochemistry. Pyrosequencing (Minimal sequence 200 nucleotide reads) revealed 80% of all sequences could be assigned to a total of 819 taxa based on default parameter of Classifier software. The phylogenetic core in Cancer individuals was different from that in Normal individuals according to the PCA analysis, with trends towards differences in the dominant and subdominant families of bacteria. Consequently, All-bacteria [log10 (bacteria/g of stool)] in Normal, and Cancer individuals were similar [11.88±0.35, and 11.80±0.56, respectively, (P = 0.16)], according to qPCR values whereas among all dominant and subdominant species only those of Bacteroides/Prevotella were higher (All bacteria-specific bacterium; P = 0.009) in Cancer (-1.04±0.55) than in Normal (-1.40±0.83) individuals. IL17 immunoreactive cells were significantly expressed more in the normal mucosa of cancer patients than in those with normal colonoscopy. This is the first large series to demonstrate a composition change in the microbiota of colon cancer patients with possible impact on mucosal immune response. These data open new filed for mass screening and pathophysiology investigations.
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64.8
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12.3
作者：
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