Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.
Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.
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DOI：
10.1038/s41598-018-20226-2
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发表时间：
2018-02-01
影响因子：
4.6
通讯作者：
Tsutsui K
中科院分区：
文献类型：
--
作者：
Nozaki M;Haraguchi S;Miyazaki T;Shigeta D;Kano N;Lei XF;Kim-Kaneyama JR;Minakata H;Miyazaki A;Tsutsui K
COS-7（具有SV 40基因的CV-1）细胞被称为非类固醇生成细胞，因为它们来源于肾细胞，而肾被定义为非类固醇生成器官。因此，COS-7细胞用于转染实验以分析包括类固醇在内的功能分子的作用。然而，初步研究表明COS-7细胞将[3 H]睾酮代谢为[3 H]雄烯二酮。这些结果表明COS-7细胞能够代谢类固醇。因此，本研究探讨类固醇合成酶的表达和类固醇在COS-7细胞中的代谢。RT-PCR分析表明，在该菌株中，细胞色素P450侧链裂解酶、3β-羟基类固醇脱氢酶/Δ5-Δ4异构酶、细胞色素P450 7α-羟化酶、细胞色素P450 17α-羟化酶/17，20-裂解酶、17β-羟基类固醇脱氢酶、5α-还原酶、细胞色素P450 21-羟化酶、细胞色素P450 11β-羟化酶、和细胞色素P450芳香化酶。此外，类固醇生成酶3β-HSD、P4507α，5α-还原酶、P450 c17、P450 c21、P450 c11 β和17β-HSD在培养的COS-7细胞中积极代谢各种类固醇。最后，我们证明了17β-HSD对雄烯二酮形成的活性大于其他类固醇生成酶的活性。我们的研究结果提供了新的证据，COS-7细胞表达一系列类固醇生成酶的mRNA和积极代谢各种类固醇。
The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids. However, a preliminary study suggested that COS-7 cells metabolize [3H]testosterone to [3H]androstenedione. These results suggest that COS-7 cells are able to metabolize steroids. Therefore, the present study investigated the expression of steroidogenic enzymes and the metabolism of steroids in COS-7 cells. RT-PCR analyses demonstrated the expressions of several kinds of steroidogenic enzymes, such as cytochrome P450 side-chain cleavage enzyme, 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase, cytochrome P450 7α-hydroxylase, cytochrome P450 17α-hydroxylase/17,20-lyase, 17β-hydroxysteroid dehydrogenase, 5α-reductase, cytochrome P450 21-hydroxylase, cytochrome P450 11β-hydroxylase, and cytochrome P450 aromatase in COS-7 cells. In addition, steroidogenic enzymes 3β-HSD, P4507α, 5α-reductase, P450c17, P450c21, P450c11β, and 17β-HSD actively metabolized various steroids in cultured COS-7 cells. Finally, we demonstrated that 17β-HSD activity toward androstenedione formation was greater than other steroidogenic enzyme activities. Our results provide new evidence that COS-7 cells express a series of steroidogenic enzyme mRNAs and actively metabolize a variety of steroids.
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影响因子：
4.6
作者：
Haraguchi S;Yamamoto Y;Suzuki Y;Hyung Chang J;Koyama T;Sato M;Mita M;Ueda H;Tsutsui K
通讯作者：
Tsutsui K
影响因子：
64.5
作者：
SMALE, ST;BALTIMORE, D
通讯作者：
BALTIMORE, D
影响因子：
64.5
作者：
MELLON, P;PARKER, V;MANIATIS, T
通讯作者：
MANIATIS, T
影响因子：
64.5
作者：
GLUZMAN, Y
通讯作者：
GLUZMAN, Y
影响因子：
4.8
作者：
Chai, ZL;Brereton, P;Krozowski, Z
通讯作者：
Krozowski, Z