CD16(+) Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
CD16(+) Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
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CD 16( ) 单核细胞亚群在系统性红斑狼疮中驱动 T 细胞激活和 B 细胞反应的过程中得到丰富且功能增强
DOI：
10.3389/fimmu.2016.00512
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发表时间：
2016
影响因子：
7.3
通讯作者：
Su Y
中科院分区：
文献类型：
--
作者：
Zhu H;Hu F;Sun X;Zhang X;Zhu L;Liu X;Li X;Xu L;Shi L;Gan Y;Su Y
在系统性红斑狼疮(SLE)中，CD16+单核细胞亚群在T细胞活化和B细胞反应中的作用尚未得到很好的研究。本研究旨在研究CD16+单核细胞亚群在SLE中的分布，并探讨其在T细胞活化和B细胞分化中的可能作用。用流式细胞仪检测正常人和系统性红斑狼疮患者外周血中单核细胞亚群的频率。分离单核细胞亚群，与CD_4~+T细胞、CD_(19)+B细胞共培养。然后采集T、B细胞进行不同亚群的检测，并收集上清液进行免疫球蛋白G、Ig A、Ig M或干扰素-γ、白介素17A的检测。我们的结果显示，CD16+单核细胞表现为促炎表型，细胞表面CD80、CD86、HLA-DR和CX3CR1的表达增加。进一步证明患者和HCS患者的CD16+单核细胞具有不同的细胞表面标志物特征。CD16+亚群在系统性红斑狼疮中丰富，并具有促进CD4+T细胞极化转变为Th17表型的能力。此外，CD16+单核细胞对CD19+B细胞分化为血浆B细胞和调节性B细胞有更强的作用，产生更多的Ig。这项研究表明，具有不同细胞表面标志物特征的CD16+单核细胞被丰富，并在驱动SLE患者的致病T细胞和B细胞反应中发挥关键作用。
The roles that CD16+ monocyte subset plays in T-cell activation and B-cell response have not been well studied in systemic lupus erythematosus (SLE). The present study aimed to investigate the distribution of CD16+ monocyte subsets in SLE and explore their possible roles in T-cell activation and B-cell differentiation. The frequencies of monocyte subsets in the peripheral blood of healthy controls (HCs) and patients with SLE were determined by flow cytometry. Monocyte subsets were sorted and cocultured with CD4+ T cells and CD19+ B cells. Then, T and B cells were collected for different subset detection, while the supernatants were collected for immunoglobulin G, IgA, and IgM or interferon-γ and interleukin-17A detection by enzyme-linked immunosorbent assay. Our results showed that CD16+ monocytes exhibited a proinflammatory phenotype with elevated CD80, CD86, HLA-DR, and CX3CR1 expression on the cell surface. It’s further demonstrated that CD16+ monocytes from patients and HCs shared different cell-surface marker profiles. The CD16+ subset was enriched in SLE and had an exacerbated capacity to promote CD4+ T cell polarization into a Th17 phenotype. Also, CD16+ monocytes had enhanced impacts on CD19+ B cells to differentiate into plasma B cells and regulatory B cells with more Ig production. This study demonstrated that CD16+ monocytes, characterized by different cell-surface marker profiles, were enriched and played a critical role in driving the pathogenic T- and B-cell responses in patients with SLE.
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影响因子：
4.9
作者：
Hilliard BA;Zizzo G;Ulas M;Linan MK;Schreiter J;Cohen PL
通讯作者：
Cohen PL
影响因子：
13.6
作者：
Crispín JC;Liossis SN;Kis-Toth K;Lieberman LA;Kyttaris VC;Juang YT;Tsokos GC
通讯作者：
Tsokos GC
影响因子：
5.5
作者：
Kim, Woong-Ki;Sun, Yue;Williams, Kenneth
通讯作者：
Williams, Kenneth
影响因子：
3.2
作者：
Castano, Diana;Garcia, Luis F.;Rojas, Mauricio
通讯作者：
Rojas, Mauricio
影响因子：
13.3
作者：
Burbano, C.;Vasquez, G.;Rojas, M.
通讯作者：
Rojas, M.