Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium sp. and Thraustochytriidae sp. through bioprocess optimization.
Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium sp. and Thraustochytriidae sp. through bioprocess optimization.
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新分离的裂殖壶菌菌株提高了分批发酵中二十二碳六烯酸的产量。
DOI：
10.1016/j.synbio.2018.04.001
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发表时间：
2018-06
影响因子：
4.8
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Wang Q;Ye H;Sen B;Xie Y;He Y;Park S;Wang G
甲藻富含二十二碳六烯酸(DHA，C22：6ω3)，是膳食脂肪酸的潜在来源。然而，培养条件对不同菌株的生长和脂肪酸组成的影响差别很大。两株不同的裂殖吸虫，裂殖吸虫Schizochytrium sp.PKU#MN4和鞭毛虫科(Thraustochytriidae sp.)对PKU#Mn16在不同培养条件下的生长和DHA产生特性进行了研究。尽管它们具有相似的脂肪酸组成，但PKU#MN4似乎是工业化DHA发酵的良好候选者，而PKU#Mn16对各种工艺条件表现出生长耐受性。在优化的摇瓶培养条件下，甘油的相对DHA含量分别为48.5%和49.2%。在优化的工艺条件下，采用双氧气控制策略，在5-L生物反应器中进行发酵，最高产率分别为21.0%和18.9%，产率分别为27.6 mg/L·h和31.9 mg/L·h。在本研究中，DHA的产量(g/L)分别提高了3.4倍和2.8倍。总体而言，我们的研究为高效生产富含DHA的油提供了两个thraustochytrid菌株的潜力和它们的培养条件。
Thraustochytrids, rich in docosahexaenoic acid (DHA, C22:6ω3), represent a potential source of dietary fatty acids. Yet, the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains. Two different thraustochytrid strains, Schizochytrium sp. PKU#Mn4 and Thraustochytriidae sp. PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions. Although they exhibited similar fatty acid profiles, PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions. Relative DHA content of 48.5% and 49.2% (relative to total fatty acids), respectively, were achieved on glycerol under their optimal flask culture conditions. Maximum DHA yield (Yp/x) of 21.0% and 18.9% and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained, respectively, in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy. A 3.4- and 2.8-fold improvement of DHA production (g/L), respectively, was achieved in this study. Overall, our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil.
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