Hyperbaric oxygen therapy sensitizes nimustine treatment for glioma in mice.
Hyperbaric oxygen therapy sensitizes nimustine treatment for glioma in mice.
复制标题
高压氧疗法使尼莫司汀治疗小鼠神经胶质瘤变得敏感
DOI：
10.1002/cam4.851
复制
发表时间：
2016-11
期刊：
影响因子：
4
通讯作者：
Huang, Qiang
中科院分区：
文献类型：
--
作者：
Lu, Zhaohui;Ma, Jiawei;Liu, Bing;Dai, Chungang;Xie, Tao;Ma, Xiaoyu;Li, Ming;Dong, Jun;Lan, Qing;Huang, Qiang
关键词：
尼莫司汀（ACNU）对恶性胶质瘤患者具有抗肿瘤活性。高压氧（HBO）可能会提高某些因缺氧微环境而受阻的治疗的疗效。我们检测了ACNU和HBO在带有人类胶质瘤模型的GFP转基因裸鼠中的联合作用。将接种人胶质瘤细胞SU3的小鼠随机分为4组：(A)对照组，(B) HBOT （HBO治疗）组，(C) ACNU组，(D) HBOT+ACNU组。用卡尺按规定时间间隔测量肿瘤大小；治疗28 d后处死小鼠，进行免疫组化染色和western blot分析。试验结束时，A、B、C、D组肿瘤重量分别为（P < 0.05）、6.03±1.47、4.13±1.82 （P < 0.05）、2.39±0.25 （P < 0.05）、1.43±0.38 （P < 0.01）。TNF - α、MMP9、HIF - α、VEGF、NF - κB和IL - 1β的表达与炎症细胞的浸润和肿瘤细胞的抑制率有关。高压氧治疗（HBOT）通过提高肿瘤组织氧压（PO2）和降低HIF‐1α、TNF‐α、IL‐1β、VEGF、MMP9和NF‐κB的表达水平，抑制胶质瘤细胞增殖和炎症细胞浸润，部分地对ACNU治疗有增敏作用。
Nimustine (ACNU) has antitumor activities in patients with malignant glioma. Hyperbaric oxygen (HBO) may enhance the efficacy of certain therapies that are hampered by the hypoxic microenvironment. We examined the combined effects of ACNU and HBO in a GFP transgenic nude mice bearing human glioma model. Mice inoculated with human glioma cells SU3 were randomly divided into the four groups: (A) the control group, (B) the HBOT (HBO therapy) group, (C) the ACNU group, and (D) the HBOT+ACNU group. Tumor size was measured at the indicated time intervals with a caliper; mice were sacrificed 28 days after treatment, and immunohistochemistry staining and western blot analysis were carried out. By the end of the trial, the tumor weights of groups A, B, C, and D were (P < 0.05), 6.03 ± 1.47, 4.13 ± 1.82 (P < 0.05), 2.39 ± 0.25 (P < 0.05), and 1.43 ± 0.38 (P < 0.01), respectively. The expressions of TNF‐α, MMP9, HIF‐α, VEGF, NF‐κB, and IL‐1β were associated with the infiltration of inflammatory cells and the inhibition rate of tumor cells. Hyperbaric oxygen therapy (HBOT) could inhibit glioma cell proliferation and inflammatory cell infiltration, and exert a sensitizing effect on ACNU therapy partially through enhancing oxygen pressure (PO2) in tumor tissues and lower expression levels of HIF‐1α, TNF‐α, IL‐1β, VEGF, MMP9, and NF‐κB.
登录
查看更多内容
人胶质瘤干祖细胞的内皮细胞体外转分化。
影响因子：
3.8
作者：
Zhao, Yaodong;Dong, Jun;Lan, Qing
通讯作者：
Lan, Qing
影响因子：
6
作者：
Petre, PM;Baciewicz, FA;Spears, JR
通讯作者：
Spears, JR
影响因子：
5.7
作者：
Lyu, MA;Rosenblum, MG
通讯作者：
Rosenblum, MG
某些人类肺癌的组织学结构以及对放射疗法的可能影响。
影响因子：
8.8
作者：
THOMLINSON, R H;GRAY, L H
通讯作者：
GRAY, L H
影响因子：
3
作者：
Takiguchi, N;Saito, N;Nakajima, N
通讯作者：
Nakajima, N