Conserved expression of the GPR151 receptor in habenular axonal projections of vertebrates.
Conserved expression of the GPR151 receptor in habenular axonal projections of vertebrates.
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DOI：
10.1002/cne.23664
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发表时间：
2015-02-15
影响因子：
2.5
通讯作者：
Ibanez-Tallon, Ines
中科院分区：
文献类型：
--
作者：
Broms, Jonas;Antolin-Fontes, Beatriz;Tingstrom, Anders;Ibanez-Tallon, Ines
关键词：
G protein-coupled receptorfasciculus retroflexusinterpeduncular nucleusrostromedial tegmental nucleusrhabdoid nucleusraphe nucleusMGI_MGI:3606630RGD_737929ZIRC_ZL1AB_10743815AB_10608138AB_307210AB_887876AB_887878AB_887883AB_887884AB_2079751AB_10123643AB_477560AB_261587AB_90754AB_572266AB_628299SciRes_000137
缰核是位于上丘脑中的一个遗传学上保守的脑结构。它是额叶边缘脑区域和脑干单胺能核之间信息流的主要节点，因此在解剖学和功能上理想地定位于调节情绪、动机和认知行为。因此，缰可能是至关重要的精神疾病，如成瘾和抑郁症的病理生理。在这里，我们研究了GPR151的表达模式，G偶联蛋白受体（GPCR），其mRNA已被确定为高度和特异性富集在缰核神经元通过原位杂交和翻译核糖体亲和纯化（TRAP）。在本免疫组化研究中，我们证明了显着的和高度特异性的表达GPR151蛋白在内侧和外侧缰的啮齿动物的大脑。在投射至脚间核、头内侧被盖区、横纹肌样核、中脑中缝核和背侧被盖核的输出缰纤维中也观察到特异性表达。使用共聚焦显微镜和定量共定位分析，我们发现，GPR151表达轴突和终端重叠的胆碱能，P物质能和多巴胺能标记。在大鼠、小鼠和斑马鱼脑中观察到几乎相同的表达模式。我们的数据表明，GPR151是高度保守的，具体的一个细分的缰神经回路，并构成了一个有前途的新的目标精神药物开发。
The habenula is a phylogenetically conserved brain structure in the epithalamus. It is a major node in the information flow between fronto-limbic brain regions and monoaminergic brainstem nuclei, thus anatomically and functionally ideally positioned to regulate emotional, motivational and cognitive behaviors. Consequently, the habenula may be critically important in the pathophysiology of psychiatric disorders such as addiction and depression. Here we investigated the expression pattern of GPR151, a G coupled-protein receptor (GPCR), whose mRNA has been identified as highly and specifically enriched in habenular neurons by in situ hybridization and Translating Ribosome Affinity Purification (TRAP). In the present immunohistochemical study we demonstrate a pronounced and highly specific expression of the GPR151 protein in the medial and lateral habenula of rodent brain. Specific expression was also seen in efferent habenular fibers projecting to the interpeduncular nucleus, the rostromedial tegmental area, the rhabdoid nucleus, the mesencephalic raphe nuclei and the dorsal tegmental nucleus. Using confocal microscopy and quantitative colocalization analysis we found that GPR151 expressing axons and terminals overlap with cholinergic, substance P-ergic and glutamatergic markers. Virtually identical expression pattern was observed in rat, mouse and zebrafish brains. Our data demonstrate that GPR151 is highly conserved, specific for a subdivision of the habenular neurocircuitry, and constitutes a promising novel target for psychiatric drug development.
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缰核：脊椎动物大脑中保守的不对称中继站。
DOI：
10.1098/rstb.2008.0213
发表时间：
2009-04-12
影响因子：
6.3
作者：
Bianco, Isaac H.;Wilson, Stephen W.
通讯作者：
Wilson, Stephen W.
影响因子：
2.5
作者：
Balcita-Pedicino, Judith Joyce;Omelchenko, Natalia;Bell, Roland;Sesack, Susan R.
通讯作者：
Sesack, Susan R.
DOI：
10.1073/pnas.76.7.3532
发表时间：
1979-01-01
影响因子：
11.1
作者：
CUELLO, AC;GALFRE, G;MILSTEIN, C
通讯作者：
MILSTEIN, C
影响因子：
3.3
作者：
Ferraro, G;Montalbano, ME;LaGrutta, V
通讯作者：
LaGrutta, V
影响因子：
3.3
作者：
CONTESTABILE, A;VILLANI, L;FONNUM, F
通讯作者：
FONNUM, F