The Basic Protein of CNS Myelin: Its Structure and Ligand Binding
The Basic Protein of CNS Myelin: Its Structure and Ligand Binding
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中枢神经系统髓磷脂的基础蛋白：其结构和配体结合
DOI：
--
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发表时间：
1992
影响因子：
4.7
通讯作者：
Ross C. Smith
中科院分区：
文献类型：
--
作者：
Ross C. Smith
考虑到本综述中提出的证据，得出以下结论： (a) 水溶液中分离的 MBP 几乎没有有序的二级或三级结构。 (b) 在这种状态下，蛋白质可以与多种疏水性和两亲性化合物缔合，这些相互作用涉及蛋白质的有限部分。 (c) 对于含有酸性脂质的系统，与双层的结合强度和伴随的蛋白质构象变化是最大的，大概是因为离子相互作用的参与。 (d) 当与酸性脂质双层结合时，MBP 将具有比其在水溶液中表现出的更加有序的二级结构，并且它可能是低聚的（可能是六聚的）。 (e) MBP 确实影响脂质聚集体的组织。它强烈影响多层纯化脂质中双层的分离，目前这必须被视为它在髓磷脂中的主要作用。我们理解 MBP 的最大障碍是缺乏可测定的生物活性。例如，与酶的情况相反，我们没有对蛋白质结构的变化或与其他分子相互作用伴随的变化进行功能测试。目前的证据表明，该蛋白质在髓磷脂内具有结构作用，并且其自身的三维结构强烈依赖于与其相关的分子。如果这幅图是正确的，那么对分离的蛋白质或重组脂质系统中的蛋白质的研究充其量可能会对其生物环境中的结构产生粗略的指导。 MBP 的结构和功能的进一步阐明可能需要等待更强大的技术的发展，用于研究与大分子聚集体（例如脂质双层）结合的蛋白质。普遍适用的方法的缺乏反映在以下事实：即使是低分辨率的结构，也仅了解少数内在膜蛋白，并且与膜表面相关的蛋白质存在更有限的信息。然而，越来越多地结合使用电子显微镜和衍射技术对脂质双层上形成的蛋白质二维阵列进行分析（Henderson 等人，1990），这为人们带来了希望，即不久之后就可以以中等分辨率研究与脂质膜结合的 MBP 的三维结构。
Consideration of the evidence presented in this review leads to the following conclusions: (a) Isolated MBP in aqueous solution has little ordered secondary or tertiary structure. (b) In this state, the protein can associate with a wide range of hydrophobic and amphiphilic compounds, these interactions involving limited sections of the protein. (c) The strength of binding to bilayers and the accompanying conformational changes in the protein are greatest for systems containing acidic lipids, presumably because of the involvement of ionic interactions. (d) When bound to bilayers of acidic lipids, MBP will have substantially more ordered secondary structure than it manifests in aqueous solution, and it is likely to be oligomeric (possibly hexameric). (e) MBP does affect the organization of lipid aggregates. It influences strongly the separation of bilayers in multilayers of purified lipids, and at present this must be viewed as its prime role within myelin. The greatest impediment to our understanding of MBP is the lack of an assayable biological activity. In contrast to the situation with enzymes, for example, we have no functional test for changes in protein structure or changes accompanying interactions with other molecules. Current evidence suggests that the protein has a structural role within myelin and that its own three-dimensional structure is strongly dependent on the molecules with which it is associated. If this picture is correct, studies of the isolated protein or of the protein in reconstituted lipid systems may yield, at best, a rough guide to the structure within its biological environment. Further clarification of the structure and function of MBP may have to await development of more powerful techniques for studying proteins bound to large molecular aggregates, such as lipid bilayers. The paucity of generally applicable methods is reflected in the fact that even low resolution structures are known for only a handful of intrinsic membrane proteins, and even more limited information exists for proteins associated with membrane surfaces. However, the increasing use of a combination of electron microscopy and diffraction on two-dimensional arrays of proteins formed on lipid bilayers (Henderson et al., 1990) offers the hope that it may not be too long before it will be possible to study at moderate resolution the three-dimensional structure of MBP bound to a lipid membrane.
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髓磷脂碱性蛋白对中性和阴离子鞘糖脂及其与二棕榈酰磷脂酰胆碱的混合物的水分散体热致行为的影响。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Maggio,B;Sturtevant,JM;Yu,RK
通讯作者：
Yu,RK
DOI：
10.1042/bj2440001
发表时间：
1987-05
期刊：
The Biochemical journal
影响因子：
--
作者：
Martin G. Low
通讯作者：
Martin G. Low
直接测定牛髓磷脂碱性蛋白中的肌醇。
DOI：
10.1042/bj2480285
发表时间：
1987
期刊：
The Biochemical journal
影响因子：
--
作者：
Smith,R;Braun,PE;Ferguson,MA;Low,MG;Sherman,WR
通讯作者：
Sherman,WR
牛髓磷脂碱性蛋白对鞘磷脂相变特性的影响。
DOI：
10.1016/0005-2736(82)90105-5
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Epand,RM;Moscarello,MA
通讯作者：
Moscarello,MA
影响因子：
5.6
作者：
HENDERSON, R;BALDWIN, JM;DOWNING, KH
通讯作者：
DOWNING, KH