Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A.
Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A.
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DOI：
10.1016/j.ydbio.2011.10.036
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发表时间：
2012-02-01
影响因子：
2.7
通讯作者：
Bruce A
中科院分区：
文献类型：
--
作者：
Du S;Draper BW;Mione M;Moens CB;Bruce A
T-box转录因子Eomesodermin（Eomes）与青蛙、鱼类和小鼠的模式化和形态发生有关。在斑马鱼中，两个Eomes同源物之一，Eomesa，已被牵连在背腹图案，epiboly和内胚层规格在实验中采用过表达，显性负结构和反义吗啉代寡核苷酸。在这里，我们报告的第一次鉴定和表征的Eomesa突变体产生的TILLING。我们发现，Eomesa有一个严格的母性的作用，在开始的外延，这涉及到圆顶的卵黄细胞到上覆胚盘。相比之下，外延进展是正常的，首次证明外延启动与进展在遗传上是分开的。卵黄细胞微管，这是所需的外包，是有缺陷的母合子eomesa mutantembryos。此外，胚盘的深层细胞比对照胚胎中的细胞更紧密地堆积，并表现出更多的水泡样突起。我们推测，圆顶延迟可能是过度稳定的卵黄细胞微管和缺陷的粘附性能或运动的深层细胞的后果。我们还表明，Eomesa是需要正常表达的内胚层标记sox 32，骨和og 9 x，但它不是必不可少的内胚层形成。
The T-box transcription factor Eomesodermin (Eomes) has been implicated in patterning and morphogenesis in frog, fish and mouse. In zebrafish, one of the two Eomes homologs, Eomesa, has been implicated in dorsal-ventral patterning, epiboly and endoderm specification in experiments employing over-expression, dominant-negative constructs and antisense morpholino oligonucleotides. Here we report for the first time the identification and characterization of an Eomesa mutant generated by TILLING. We find that Eomesa has a strictly maternal role in the initiation of epiboly, which involves doming of the yolk cell up into the overlying blastoderm. By contrast, epiboly progression is normal, demonstrating for the first time that epiboly initiation is genetically separable from progression. The yolk cell microtubules, which are required for epiboly, are defective in maternal-zygotic eomesa mutantembryos. In addition, the deep cells of the blastoderm are more tightly packed and exhibit more bleb-like protrusions than cells in control embryos. We postulate that the doming delay may be the consequence both of overly stabilized yolk cell microtubules and defects in the adhesive properties or motility of deep cells. We also show that Eomesa is required for normal expression of the endoderm markers sox32, bon and og9x; however it is not essential for endoderm formation.
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影响因子：
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作者：
Bjornson, CRR;Griffin, KJR;Kimelman, D
通讯作者：
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影响因子：
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