Gene-for-gene relationships between rice and diverse avrBs3/pthA avirulence genes in Xanthomonas oryzae pv. oryzae
Gene-for-gene relationships between rice and diverse avrBs3/pthA avirulence genes in Xanthomonas oryzae pv. oryzae
复制标题
水稻与黄单胞菌 pv 中多种 avrBs3/pthA 无毒基因之间的基因对基因关系。
DOI：
10.1111/j.1365-3059.2006.01495.x
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发表时间：
2007-02
期刊：
影响因子：
2.7
通讯作者：
Li, Y. R.
中科院分区：
文献类型：
--
作者：
Zou, L. F.;Wu, X. M.;Chen, G. Y.;Li, Y. R.
研究了黄单胞菌番茄溃疡病致病型（Xanthomonasandrogpv.）Xoo和水稻是以基因对基因的方式控制的。在这项研究中，一个359 bp的DNA片段的avrXa 3基因含有三个核定位信号（NLS）基序存在于所有成员的avrBs 3/pthA家族作为探针，以筛选基因组文库的JXO Ⅲ株Xoo。结果表明，不同的家庭成员存在于病原体基因组。avrBs 3/pthA基因出现在孤立的个体部分或簇中。将avr基因的阳性克隆转入强毒受体PXO 99 A中。在水稻近等基因系中的致病性测试证实，4个抗性（R）基因（Xa 2、Xa 3、xa 5和xa 8）与Xoo菌株JXO III基因组中的4个avr基因（avrXa 2、avrXa 3、avrxa 5和avrxa 8）相匹配。存在于粘粒p54中的avrBs 3/PthA样基因（1.7 kb）可与存在于IRBB 3中的Xa 3基因特异性相互作用，并被命名为avr/pthA 3。测序结果表明，avr/pthA 3中102 bp的重复单元只有1·5个拷贝。该基因编码的重复单元中的第12和第13个氨基酸与AvrBs 3家族的其他代表中的氨基酸的比对揭示了一种独特的重复排列，这可能有助于Xoo中无毒基因的变异。水稻亲本IR 24含有多个抗白叶枯病的R基因。
The interactions between Xanthomonas oryzae pv. oryzae (Xoo) and rice are controlled in a gene-for-gene manner. In this study, a 359 bp DNA fragment of the avrXa3 gene containing three nuclear localization signal (NLS) motifs present in all members of the avrBs3/pthA family was used as a probe to screen a genomic library of the JXOIII strain of Xoo. The results demonstrated that diverse members of the family exist in the pathogen genome. The avrBs3/pthA genes occurred at isolated individual portions or in clusters. The positive avr gene clones were transferred into the virulent recipient PXO99A. Pathogenicity tests in near isogenic lines of rice confirmed that four resistance (R) genes (Xa2, Xa3, xa5 and xa8) matched the four avr genes (avrXa2, avrXa3, avrxa5 and avrxa8) in the genome of Xoo strain JXOIII. The avrBs3/PthA-like gene (1·7 kb) present in cosmid p54, may specifically interact with the Xa3 gene present in IRBB3, and is designated avr/pthA3. Sequencing indicated that there are only 1·5 copies of the 102 bp repeat unit in avr/pthA3. Alignment of the twelfth and thirteenth amino acids in the repetitive units encoded by this gene with those in other representatives of the AvrBs3 family revealed a unique repeat arrangement which might contribute to variation in the avirulence genes in Xoo. The parental rice line IR24 was found to contain several R genes for resistance to Xoo bacterial blight.
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DOI：
10.1080/10020070412331344311
发表时间：
2004-09
期刊：
Progress in Natural Science
影响因子：
--
作者：
Ping Li;Juying Long;Y. Huang;Yan Zhang;Jinsheng Wang
通讯作者：
Ping Li;Juying Long;Y. Huang;Yan Zhang;Jinsheng Wang
DOI：
10.1038/nbt1183-784
发表时间：
1983-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
SIMON, R;PRIEFER, U;PUHLER, A
通讯作者：
PUHLER, A
影响因子：
9.5
作者：
F. White;Bing Yang;L. B. Johnson
通讯作者：
F. White;Bing Yang;L. B. Johnson
影响因子：
2.1
作者：
Dharmapuri, S;Sonti, RV
通讯作者：
Sonti, RV
影响因子：
64.8
作者：
Gu, KY;Yang, B;Yin, ZC
通讯作者：
Yin, ZC