Biological properties of dehydrated human amnion/chorion composite graft: implications for chronic wound healing.
Biological properties of dehydrated human amnion/chorion composite graft: implications for chronic wound healing.
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DOI：
10.1111/iwj.12140
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发表时间：
2013-10
影响因子：
3.1
通讯作者：
Gurtner G
中科院分区：
文献类型：
--
作者：
Koob TJ;Rennert R;Zabek N;Massee M;Lim JJ;Temenoff JS;Li WW;Gurtner G
来自胎盘的人类羊膜/绒毛膜组织富含已知可促进伤口愈合的细胞因子和生长因子；然而，在加工过程中保持治疗性同种异体移植物的生物活性仍然是一个挑战。在这项研究中，评估了 PURION®（MiMedx，Marietta，GA）处理的脱水人羊膜/绒毛膜组织同种异体移植物（dHACM、EpiFix®、MiMedx）中生长因子、白细胞介素 (IL) 和金属蛋白酶组织抑制剂 (TIMP) 的存在情况。对 dHACM 样本进行酶联免疫吸附测定 (ELISA)，结果显示以下生长因子的可定量水平：血小板衍生生长因子-AA (PDGF-AA)、PDGF-BB、转化生长因子 α (TGFα)、TGFβ1、碱性成纤维细胞生长因子 (bFGF)、表皮生长因子 (EGF)、 胎盘生长因子（PLGF）和粒细胞集落刺激因子（GCSF）。 ELISA 测定还证实了 IL-4、6、8 和 10 以及 TIMP 1、2 和 4 的存在。此外，同种异体移植物中生长因子到盐水中的相对洗脱率为 4% 至 62%，表明同种异体移植物中存在这些化合物的结合和未结合部分。 dHACM 保留了在体外引起人真皮成纤维细胞增殖和人间充质干细胞 (MSC) 迁移的生物活性。体内小鼠模型表明，在皮瓣模型中进行测试时，dHACM 会导致间充质祖细胞募集到植入部位。体外和体内实验的结果清楚地表明，dHACM 含有一种或多种能够刺激 MSC 迁移和招募的可溶性因子。总之，经过 PURION® 处理的 dHACM 保留了与伤口愈合相关的生物活性，包括对与伤口愈合密切相关的四个不同且关键的生理过程产生积极影响的潜力：细胞增殖、炎症、金属蛋白酶活性和祖细胞的募集。这表明 dHACM 在用于伤口愈合应用时具有旁分泌作用机制。
Human amnion/chorion tissue derived from the placenta is rich in cytokines and growth factors known to promote wound healing; however, preservation of the biological activities of therapeutic allografts during processing remains a challenge. In this study, PURION® (MiMedx, Marietta, GA) processed dehydrated human amnion/chorion tissue allografts (dHACM, EpiFix®, MiMedx) were evaluated for the presence of growth factors, interleukins (ILs) and tissue inhibitors of metalloproteinases (TIMPs). Enzyme‐linked immunosorbent assays (ELISA) were performed on samples of dHACM and showed quantifiable levels of the following growth factors: platelet‐derived growth factor‐AA (PDGF‐AA), PDGF‐BB, transforming growth factor α (TGFα), TGFβ1, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), placental growth factor (PLGF) and granulocyte colony‐stimulating factor (GCSF). The ELISA assays also confirmed the presence of IL‐4, 6, 8 and 10, and TIMP 1, 2 and 4. Moreover, the relative elution of growth factors into saline from the allograft ranged from 4% to 62%, indicating that there are bound and unbound fractions of these compounds within the allograft. dHACM retained biological activities that cause human dermal fibroblast proliferation and migration of human mesenchymal stem cells (MSCs) in vitro. An in vivo mouse model showed that dHACM when tested in a skin flap model caused mesenchymal progenitor cell recruitment to the site of implantation. The results from both the in vitro and in vivo experiments clearly established that dHACM contains one or more soluble factors capable of stimulating MSC migration and recruitment. In summary, PURION® processed dHACM retains its biological activities related to wound healing, including the potential to positively affect four distinct and pivotal physiological processes intimately involved in wound healing: cell proliferation, inflammation, metalloproteinase activity and recruitment of progenitor cells. This suggests a paracrine mechanism of action for dHACM when used for wound healing applications.
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影响因子：
3.1
作者：
Sheikh ES;Sheikh ES;Fetterolf DE
通讯作者：
Fetterolf DE
影响因子：
1.5
作者：
Russo, Alessandra;Bonci, Paola;Bonci, Paolo
通讯作者：
Bonci, Paolo
影响因子：
15.9
作者：
LYNCH, SE;COLVIN, RB;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
DOI：
10.1111/j.1524-475x.2011.00673.x
发表时间：
2011-03
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
Schultz GS;Davidson JM;Kirsner RS;Bornstein P;Herman IM
通讯作者：
Herman IM
影响因子：
6
作者：
Rodriguez-Menocal, Luis;Salgado, Marcela;Van Badiavas, Evangelos
通讯作者：
Van Badiavas, Evangelos