Defining the Syrian hamster as a highly susceptible preclinical model for SARS-CoV-2 infection.
Defining the Syrian hamster as a highly susceptible preclinical model for SARS-CoV-2 infection.
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DOI：
10.1080/22221751.2020.1858177
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发表时间：
2020-12
影响因子：
13.2
通讯作者：
Feldmann H
中科院分区：
文献类型：
--
作者：
Rosenke K;Meade-White K;Letko M;Clancy C;Hansen F;Liu Y;Okumura A;Tang-Huau TL;Li R;Saturday G;Feldmann F;Scott D;Wang Z;Munster V;Jarvis MA;Feldmann H
自2019年底出现后，SARS-CoV-2迅速成为大流行，目前导致全球数百万人感染和数十万人死亡。目前还没有批准的疫苗来阻止SARS-CoV-2的传播，也只有极少数的治疗方案可用于管理新冠肺炎患者。对于临床前对策的开发，可靠和具有良好特征的小动物疾病模型将是至关重要的。在这里，我们发现，经鼻接种SARS-CoV-2给叙利亚仓鼠持续引起中度的支气管间质性肺炎，具有较高的病毒肺负荷和广泛的病毒脱落，但动物只表现出一过性的轻度疾病。我们确定的感染剂量50只是5个感染颗粒，使叙利亚仓鼠成为SARS-CoV-2感染的高度敏感模型。仓鼠的年龄和性别对疾病的严重程度或感染过程都没有任何影响。最后，在白介素2受体伽马链敲除仓鼠中的病毒持续时间延长表明SARS-CoV-2对适应性免疫控制的敏感性。综上所述，叙利亚仓鼠对SARS-CoV-2高度易感，是非常适合制定新冠肺炎对策的感染模型。
Following emergence in late 2019, SARS-CoV-2 rapidly became pandemic and is presently responsible for millions of infections and hundreds of thousands of deaths worldwide. There is currently no approved vaccine to halt the spread of SARS-CoV-2 and only very few treatment options are available to manage COVID-19 patients. For development of preclinical countermeasures, reliable and well-characterized small animal disease models will be of paramount importance. Here we show that intranasal inoculation of SARS-CoV-2 into Syrian hamsters consistently caused moderate broncho-interstitial pneumonia, with high viral lung loads and extensive virus shedding, but animals only displayed transient mild disease. We determined the infectious dose 50 to be only five infectious particles, making the Syrian hamster a highly susceptible model for SARS-CoV-2 infection. Neither hamster age nor sex had any impact on the severity of disease or course of infection. Finally, prolonged viral persistence in interleukin 2 receptor gamma chain knockout hamsters revealed susceptibility of SARS-CoV-2 to adaptive immune control. In conclusion, the Syrian hamster is highly susceptible to SARS-CoV-2 making it a very suitable infection model for COVID-19 countermeasure development.
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影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
30.3
作者：
Kim, Young-Il;Kim, Seong-Gyu;Choi, Young Ki
通讯作者：
Choi, Young Ki
影响因子：
5.4
作者：
McCray, Paul B., Jr.;Pewe, Lecia;Perlman, Stanley
通讯作者：
Perlman, Stanley
影响因子：
11.8
作者：
Harcourt, Jennifer;Tamin, Azaibi;Thornburg, Natalie J.
通讯作者：
Thornburg, Natalie J.
DOI：
10.1016/j.ijantimicag.2020.105949
发表时间：
2020-07-01
影响因子：
10.8
作者：
Gautret, Philippe;Lagier, Jean-Christophe;Raoult, Didier
通讯作者：
Raoult, Didier