Cell specificity of magnetic cell seeding approach to hydrogel colonization.
Cell specificity of magnetic cell seeding approach to hydrogel colonization.
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磁性细胞接种方法水凝胶定植的细胞特异性
DOI：
10.1002/jbm.a.36147
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Cicha I
中科院分区：
文献类型：
--
作者：
Singh R;Wieser A;Reakasame S;Detsch R;Dietel B;Alexiou C;Boccaccini AR;Cicha I
组织工程支架需要细胞的有效定植。超顺磁性氧化铁纳米颗粒 (SPION) 可以通过磁性细胞接种增强细胞对基质的粘附。我们研究了使用负载 SPION 的成纤维细胞和内皮细胞 (EC) 改善细胞在不同藻酸盐基水凝胶上附着和生长的可能性。制备了含有纯海藻酸盐（Alg）、与明胶交联的海藻酸二醛（ADA-G）以及与G或丝素蛋白（SF）混合的Alg的水凝胶。将负载有 SPION 的内皮细胞和成纤维细胞接种在水凝胶上并在前 24 小时内在磁场存在下生长长达 7 天。比较了磁性接种细胞与传统接种细胞的细胞形态（荧光染色）和代谢活性（WST-8 测定）。 EC 的磁力播种改善了它们在 Alg/G 水凝胶表面上的初始附着和进一步生长。然而，即使采用磁性细胞接种，我们也没有实现 ADA-G 膜与 EC 的有效和稳定的定植。成纤维细胞在 ADA-G 和 Alg/SF 上显示出良好的初始定植和生长。磁性细胞接种进一步显着增强了这种效果。在纯 Alg 上，与传统种子细胞相比，磁性种子细胞的初始附着和扩散得到显着改善，但效果是短暂的，并随着磁力的停止而逐渐减弱。我们的结果表明，磁性接种以细胞特异性的方式提高了初始细胞附着到水凝胶表面的强度和均匀性，这可能对组织工程应用的结果发挥决定性作用。 © 2017 Wiley periodicals, Inc. J Biomed Mater Res 第 A 部分：105A：2948–2956，2017 年。
Tissue‐engineered scaffolds require an effective colonization with cells. Superparamagnetic iron oxide nanoparticles (SPIONs) can enhance cell adhesion to matrices by magnetic cell seeding. We investigated the possibility of improving cell attachment and growth on different alginate‐based hydrogels using fibroblasts and endothelial cells (ECs) loaded with SPIONs. Hydrogels containing pure alginate (Alg), alginate dialdehyde crosslinked with gelatin (ADA‐G) and Alg blended with G or silk fibroin (SF) were prepared. Endothelial cells and fibroblasts loaded with SPIONs were seeded and grown on hydrogels for up to 7 days, in the presence of magnetic field during the first 24 h. Cell morphology (fluorescent staining) and metabolic activity (WST‐8 assay) of magnetically‐seeded versus conventionally seeded cells were compared. Magnetic seeding of ECs improved their initial attachment and further growth on Alg/G hydrogel surfaces. However, we did not achieve an efficient and stable colonization of ADA‐G films with ECs even with magnetic cell seeding. Fibroblast showed good initial colonization and growth on ADA‐G and on Alg/SF. This effect was further significantly enhanced by magnetic cell seeding. On pure Alg, initial attachment and spreading of magnetically‐seeded cells was dramatically improved compared to conventionally‐seeded cells, but the effect was transient and diminished gradually with the cessation of magnetic force. Our results demonstrate that magnetic seeding improves the strength and uniformity of initial cell attachment to hydrogel surface in cell‐specific manner, which may play a decisive role for the outcome in tissue engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2948–2956, 2017.
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