Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress.
Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress.
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DOI：
10.3389/fpls.2015.00353
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发表时间：
2015
影响因子：
5.6
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Yan Y;Takáč T;Li X;Chen H;Wang Y;Xu E;Xie L;Su Z;Šamaj J;Xu C
关于植物低温反应过程中阿拉伯半乳聚糖蛋白（AGPs）在植物器官和组织中的空间分布的信息有限。本研究采用免疫技术，利用17种针对不同AGP表位的单克隆抗体，研究了两种不同抗寒性基因型香蕉叶片和根中AGP的细胞外分布及其在低温胁迫下的变化。还测试了香蕉叶中总经典AGPs的变化。结果表明，JIM4、JIM14、JIM16和CCRC-M32抗体识别的AGP表位主要分布在叶脉中，而JIM8、JIM13、JIM15和PN16.4B4抗体识别的AGP表位主要分布在质层中。LM2、LM14和MAC207抗体识别的表位分布在表皮和叶肉细胞中。两种基因型在低温处理下均积累了经典AGPs，耐冷基因型在各温度处理下均具有较高的经典AGPs。低温敏感基因型的JIM4和JIM16表位丰度在低温处理后略有下降，耐冷基因型则相反。与敏感基因型相比，耐药基因型诱导LM2-和lm14免疫反应性AGPs的积累，特别是在韧皮部和叶肉细胞中。因此，这些表位可能在香蕉的LT耐受性中起重要作用。不同AGP成分在香蕉根中的分布也存在差异。一般来说，香蕉根在低温处理下开始积累AGPs的时间要早于叶片。耐冷基因型对照根中MAC207和JIM13抗体识别的AGPs水平高于冷敏感基因型对照根。耐冷基因型对JIM13抗体表现出较高的免疫反应性。这些结果表明，香蕉抗寒性可能与几种agp有关。
Information on the spatial distribution of arabinogalactan proteins (AGPs) in plant organs and tissues during plant reactions to low temperature (LT) is limited. In this study, the extracellular distribution of AGPs in banana leaves and roots, and their changes under LT stress were investigated in two genotypes differing in chilling tolerance, by immuno-techniques using 17 monoclonal antibodies against different AGP epitopes. Changes in total classical AGPs in banana leaves were also tested. The results showed that AGP epitopes recognized by JIM4, JIM14, JIM16, and CCRC-M32 antibodies were primarily distributed in leaf veins, while those recognized by JIM8, JIM13, JIM15, and PN16.4B4 antibodies exhibited predominant sclerenchymal localization. Epitopes recognized by LM2, LM14, and MAC207 antibodies were distributed in both epidermal and mesophyll cells. Both genotypes accumulated classical AGPs in leaves under LT treatment, and the chilling tolerant genotype contained higher classical AGPs at each temperature treatment. The abundance of JIM4 and JIM16 epitopes in the chilling-sensitive genotype decreased slightly after LT treatment, and this trend was opposite for the tolerant one. LT induced accumulation of LM2- and LM14-immunoreactive AGPs in the tolerant genotype compared to the sensitive one, especially in phloem and mesophyll cells. These epitopes thus might play important roles in banana LT tolerance. Different AGP components also showed differential distribution patterns in banana roots. In general, banana roots started to accumulate AGPs under LT treatment earlier than leaves. The levels of AGPs recognized by MAC207 and JIM13 antibodies in the control roots of the tolerant genotype were higher than in the chilling sensitive one. Furthermore, the chilling tolerant genotype showed high immuno-reactivity against JIM13 antibody. These results indicate that several AGPs are likely involved in banana tolerance to chilling injury.
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影响因子：
5.6
作者：
Knoch E;Dilokpimol A;Geshi N
通讯作者：
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影响因子：
6.4
作者：
Kozbial, PZ;Jerzmanowski, A;Kacperska, A
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影响因子：
5.2
作者：
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影响因子：
6.9
作者：
Ma H;Zhao J
通讯作者：
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影响因子：
5.3
作者：
Losada JM;Herrero M
通讯作者：
Herrero M