Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling.
Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling.
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通过数字基因表达谱鉴定交配和处女家蚕信息素腺中差异表达的基因
DOI：
10.1371/journal.pone.0111003
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
An S
中科院分区：
文献类型：
--
作者：
Zhang S;Liu X;Zhu B;Yin X;Du M;Song Q;An S
交配降低雌蛾的感受性，终止性信息素的产生。虽然在阐明交配调控的信息素生物合成激活神经肽（PBAN）分泌失活方面取得了重大进展，但对交配诱导的信息素腺体（PGs）基因表达谱知之甚少。本研究通过数字基因表达谱（DGE）和随后的RNA干扰（RNAi）技术对家蚕交配相关基因进行鉴定，以阐明家蚕生殖细胞中交配调控基因表达的分子机制。结果从交配和未交配雌性的PG中构建了8个DGE文库：交配1h（M1）/未交配（V1）PG、交配3 h（M3）/未交配（V3）PG、交配24 h（M24）/未交配（V24）PG和交配48 h（M48）/未交配（V48）PG（M48和V48）。这些文库用于研究受交配影响的基因表达谱。DGE分析揭示了一系列的基因表现出差异表达的每组交配和处女的女性样品，包括免疫相关基因，性信息素合成相关基因，保幼激素（JH）信号相关基因等，最有趣的是，JH信号被发现激活交配。应用JH模拟物甲氧普烯对新羽化的处女雌虫进行处理，导致性信息素的产生显著减少。RNAi介导的敲除雌蛹中的JH受体基因Methoprene tolerant 1（Met 1）导致成熟雌虫性信息素产生的显著减少，表明JH在性信息素合成中的重要性。结论发现了一系列与交配反应相关的PGs差异表达基因。这项研究提高了我们的理解JH信号的作用交配引发终止性信息素的生产。
Background Mating decreases female receptivity and terminates sex pheromone production in moths. Although significant progress has been made in elucidating the mating-regulated inactivation of pheromone biosynthesis-activating neuropeptide (PBAN) secretion, little is known about the mating induced gene expression profiles in pheromone glands (PGs). In this study, the associated genes involved in Bombyx mori mating were identified through digital gene expression (DGE) profiling and subsequent RNA interference (RNAi) to elucidate the molecular mechanisms underlying the mating-regulated gene expression in PGs. Results Eight DGE libraries were constructed from the PGs of mated and virgin females: 1 h mating (M1)/virgin (V1) PGs, 3 h mating (M3)/virgin (V3) PGs, 24 h mating (M24)/virgin (V24) PGs and 48 h mating (M48)/virgin (V48) PGs (M48 and V48). These libraries were used to investigate the gene expression profiles affected by mating. DGE profiling revealed a series of genes showing differential expression in each set of mated and virgin female samples, including immune-associated genes, sex pheromone synthesis-associated genes, juvenile hormone (JH) signal-associated genes, etc. Most interestingly, JH signal was found to be activated by mating. Application of the JH mimics, methoprene to the newly-emerged virgin females leaded to the significant reduction of sex pheromone production. RNAi-mediated knockdown of putative JH receptor gene, Methoprene tolerant 1 (Met1), in female pupa resulted in a significant decrease in sex pheromone production in mature females, suggesting the importance of JH in sex pheromone synthesis. Conclusion A series of differentially expressed genes in PGs in response to mating was identified. This study improves our understanding of the role of JH signaling on the mating-elicited termination of sex pheromone production.
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影响因子：
3.7
作者：
Du M;Yin X;Zhang S;Zhu B;Song Q;An S
通讯作者：
An S
影响因子：
4
作者：
An, Shi-Heng;Du, Meng-Fang;Yin, Xin-Ming
通讯作者：
Yin, Xin-Ming
影响因子：
7
作者：
Morrissy, A. Sorana;Morin, Ryan D.;Marra, Marco A.
通讯作者：
Marra, Marco A.
影响因子：
9.2
作者：
Peng, J;Zipperlen, P;Kubli, E
通讯作者：
Kubli, E
DOI：
10.1073/pnas.1318119110
发表时间：
2013-11-05
影响因子：
11.1
作者：
Bilen, Julide;Atallah, Jade;Riddiford, Lynn M.
通讯作者：
Riddiford, Lynn M.