Direct inhibition of phosphate transport by immune signaling in Arabidopsis.
Direct inhibition of phosphate transport by immune signaling in Arabidopsis.
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DOI：
10.1016/j.cub.2021.11.063
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发表时间：
2022-01-24
期刊：
影响因子：
--
通讯作者：
Zipfel C
中科院分区：
文献类型：
--
作者：
Dindas J;DeFalco TA;Yu G;Zhang L;David P;Bjornson M;Thibaud MC;Custódio V;Castrillo G;Nussaume L;Macho AP;Zipfel C
土壤中无机正磷酸盐（PO 43 −，Pi）的有效性是植物生长和健康的关键决定因素。植物通过磷酸盐饥饿反应（PSR）的遗传网络驱动的转录和翻译后变化来适应质膜（PM）上磷酸盐转运蛋白1（PHOSPHATE TRANSPORTER 1，PHT 1）家族的H+-偶联磷酸盐转运蛋白的丰度，从而调节其根系吸收无机磷的能力。越来越多的证据表明，植物通过与有益微生物的结合来整合免疫反应以缓解磷饥饿胁迫。这种磷酸盐转运是否以及如何在免疫应答激活后被调节还没有被表征。为了解决这个问题，我们首先开发了定量分析的基础上的变化，在电力PM电位测量积极的Pi运输在根中的真实的时间。通过将微电极插入凸出的根毛中，我们能够确定完整的拟南芥（以下简称拟南芥）幼苗中磷酸盐转运的关键特征。观察到的快速Pi诱导的去极化依赖于主要磷酸盐转运蛋白PHT 1的活性;4。值得注意的是，我们观察到这种PHT 1;4介导的磷酸盐摄取在模式触发的免疫激活后被抑制。这种抑制依赖于受体样细胞质激酶肉毒杆菌诱导激酶1（BIK 1）和PBS 1样激酶1（PBL 1），这两个磷酸化的PHT 1;4。作为一个必然的结果，这种负调节磷酸盐运输的免疫信号，我们发现，PHT 1;4介导的磷酸盐吸收通常负调节抗细菌免疫的根。总的来说，我们的研究结果揭示了植物免疫和磷酸盐稳态之间的联系机制，BIK 1/PBL 1在这两个重要途径之间提供了分子整合点。实时，PHT 1介导的Pi运输可以通过PM去极化在根中测量免疫激活以BIK 1/PBL 1依赖的方式抑制Pi运输PHT 1是BIK 1和PBL 1的直接底物Pi摄取调节抗细菌免疫和根微生物组磷酸盐（Pi）是植物生长和适应性的关键决定因素。Dindas，DeFalco，等.表明根中的免疫激活积极抑制Pi吸收。这种抑制作用是通过中枢免疫激酶BIK 1和PBL 1直接磷酸化关键Pi转运蛋白来实现的，这导致了抗菌防御的增加。
Soil availability of inorganic ortho-phosphate (PO43−, Pi) is a key determinant of plant growth and fitness. Plants regulate the capacity of their roots to take up inorganic phosphate by adapting the abundance of H+-coupled phosphate transporters of the PHOSPHATE TRANSPORTER 1 (PHT1) family at the plasma membrane (PM) through transcriptional and post-translational changes driven by the genetic network of the phosphate starvation response (PSR). Increasing evidence also shows that plants integrate immune responses to alleviate phosphate starvation stress through the association with beneficial microbes. Whether and how such phosphate transport is regulated upon activation of immune responses is yet uncharacterized. To address this question, we first developed quantitative assays based on changes in the electrical PM potential to measure active Pi transport in roots in real time. By inserting micro-electrodes into bulging root hairs, we were able to determine key characteristics of phosphate transport in intact Arabidopsis thaliana (hereafter Arabidopsis) seedlings. The fast Pi-induced depolarization observed was dependent on the activity of the major phosphate transporter PHT1;4. Notably, we observed that this PHT1;4-mediated phosphate uptake is repressed upon activation of pattern-triggered immunity. This inhibition depended on the receptor-like cytoplasmic kinases BOTRYTIS-INDUCED KINASE 1 (BIK1) and PBS1-LIKE KINASE 1 (PBL1), which both phosphorylated PHT1;4. As a corollary to this negative regulation of phosphate transport by immune signaling, we found that PHT1;4-mediated phosphate uptake normally negatively regulates anti-bacterial immunity in roots. Collectively, our results reveal a mechanism linking plant immunity and phosphate homeostasis, with BIK1/PBL1 providing a molecular integration point between these two important pathways. Real-time, PHT1-mediated Pi transport can be measured in roots via PM depolarization Immune activation represses Pi transport in a BIK1/PBL1-dependent manner PHT1s are direct substrates of BIK1 and PBL1 Pi uptake modulates anti-bacterial immunity and the root microbiome Phosphate (Pi) is a crucial determinant of plant growth and fitness. Dindas, DeFalco, et al. show that immune activation in roots actively represses Pi uptake. This inhibition is achieved via direct phosphorylation of key Pi transporters by the central immune kinases BIK1 and PBL1, which leads to increased anti-bacterial defense.
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影响因子：
18
作者：
Bjornson M;Pimprikar P;Nürnberger T;Zipfel C
通讯作者：
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影响因子：
11.6
作者：
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影响因子：
16.6
作者：
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通讯作者：
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影响因子：
16.6
作者：
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通讯作者：
O'Connell RJ
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP