Photothermal therapy of cancer cells mediated by blue hydrogel nanoparticles.
Photothermal therapy of cancer cells mediated by blue hydrogel nanoparticles.
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DOI：
10.2217/nnm.12.190
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Kopelman R
中科院分区：
文献类型：
--
作者：
Curry T;Epstein T;Smith R;Kopelman R
本研究的目的是在体外研究生物相容性、无毒且细胞特异性的可靶向水凝胶纳米颗粒 (NP) 作为癌细胞光热疗法 (PTT) 候选物的效用，该纳米颗粒将考马斯亮蓝 G 染料（Sigma-Aldrich，密苏里州，美国）共价连接到其聚丙烯酰胺基质中。采用反胶束微乳液聚合方法制备了考马斯亮蓝 G 染料共价连接到聚丙烯酰胺基质中的水凝胶纳米粒子，直径为 80-95 nm，吸光度值为 0.52。使用廉价、便携式、发光二极管阵列光源（590 nm，25 mW/cm2）在 37°C 下实现 PTT 诱导的高热/热解。具有考马斯亮蓝 G 染料的水凝胶纳米粒子连接到其聚丙烯酰胺基质中，在不同的纳米粒子浓度和处理时间下，可以有效地在永生化人宫颈癌细胞系 (HeLa) 细胞中引起 PTT 诱导的热解。这些多功能颗粒此前已用于癌症研究、神经胶质瘤手术和光声成像研究中进行描绘。 PTT功能的增加将使癌症医学的三管齐下的治疗诊断方法成为可能，例如利用术中光声成像和术中PTT引导肿瘤手术。
The aim of this study was to investigate in vitro the utility of biologically compatible, nontoxic and cell-specific targetable hydrogel nanoparticles (NPs), which have Coomassie® Brilliant Blue G dye (Sigma-Aldrich, MO, USA) covalently linked into their polyacrylamide matrix, as candidates for photothermal therapy (PTT) of cancer cells. Hydrogel NPs with Coomassie Brilliant Blue G dye covalently linked into their polyacrylamide matrix were fabricated using a reverse micelle microemulsion polymerization method and were found to be 80–95 nm in diameter, with an absorbance value of 0.52. PTT-induced hyperthermia/thermolysis was achieved at 37°C using an inexpensive, portable, light-emitting diode array light source (590 nm, 25 mW/cm2). Hydrogel NPs with Coomassie Brilliant Blue G dye linked into their polyacrylamide matrix are effective in causing PTT-induced thermolysis in immortalized human cervical cancer cell line (HeLa) cells for varying NP concentrations and treatment times. These multifunctional particles have previously been used in cancer studies to enable delineation, for glioma surgery and in photoacoustic imaging studies. The addition of the PTT function would enable a three-pronged theranostic approach to cancer medicine, such as guided tumor surgery with intra-operative photoacoustic imaging and intra-operative PTT.
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影响因子：
4.8
作者：
Orringer DA;Chen T;Huang DL;Armstead WM;Hoff BA;Koo YE;Keep RF;Philbert MA;Kopelman R;Sagher O
通讯作者：
Sagher O
影响因子：
2.7
作者：
Kopelman, R;Koo, YEL;Ross, BD
通讯作者：
Ross, BD
影响因子：
9.7
作者：
Boca, Sanda C.;Potara, Monica;Astilean, Simion
通讯作者：
Astilean, Simion
影响因子：
3.5
作者：
Kirui, Dickson K.;Rey, Diego A.;Batt, Carl A.
通讯作者：
Batt, Carl A.
影响因子：
2.4
作者：
Wang, Shouyan;Fan, Wenzhe;Kim, Gwangseong;Hah, Hoe Jin;Lee, Yong-Eun Koo;Kopelman, Raoul;Ethirajan, Manivannan;Gupta, Anurag;Goswami, Lalit N.;Pera, Paula;Morgan, Janet;Pandey, Ravindra K.
通讯作者：
Pandey, Ravindra K.