Studying osteocytes within their environment.
Studying osteocytes within their environment.
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DOI：
10.1016/j.bone.2013.01.004
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发表时间：
2013-06
期刊：
影响因子：
4.1
通讯作者：
Mueller, Ralph
中科院分区：
文献类型：
--
作者：
Webster, Duncan J.;Schneider, Philipp;Dallas, Sarah L.;Mueller, Ralph
骨细胞是骨矿化基质中的力学感受器，控制着负荷诱导的骨适应。由于它们的不可及性，已经证明生成支持这一假设的定量体内实验数据具有挑战性。最近的进展，在原位成像，无论是在非生活和生活标本，提供了新的见解骨细胞在骨骼中的作用。结合使用体内模型从机械刺激的骨细胞中检索生化信息，定量实验数据现在变得可用，这导致对骨细胞功能的更准确理解。考虑到这一点，我们在这里回顾了i）最先进的离体成像模式，能够在3D中精确捕获骨细胞微观和超微结构，ii）活细胞成像技术，能够在空间和时间上跟踪结构形态和细胞分化，（三）当与最新的生物化学测定和微流体成像技术相结合时，体内模型可以提供对生物学特性的进一步了解。骨细胞的功能。
It is widely hypothesized that osteocytes are the mechano-sensors residing in bone’s mineralized matrix which control load induced bone adaptation. Owing to their inaccessibility it has proved challenging to generate quantitative in vivo experimental data which supports this hypothesis. Recent advances in in situ imaging, both in non-living and living specimens, have provided new insights into the role of osteocytes in the skeleton. Combined with the retrieval of biochemical information from mechanically stimulated osteocytes using in vivo models, quantitative experimental data is now becoming available which is leading to a more accurate understanding of osteocyte function. With this in mind, here we review i) State of the art ex vivo imaging modalities which are able to precisely capture osteocyte micro and ultrastructure in 3D, ii) Live cell imaging techniques which are able to track structural morphology and cellular differentiation in both space and time, iii) In vivo models which when combined with the latest biochemical assays and microfluidic imaging techniques can provide further insight on the biological function of osteocytes.
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影响因子：
4.1
作者：
Ciani, Cesare;Doty, Stephen B.;Fritton, Susannah R.
通讯作者：
Fritton, Susannah R.
影响因子：
2.4
作者：
Ascenzi, Maria-Grazia;Gill, Jaya;Lomovtsev, Alexander
通讯作者：
Lomovtsev, Alexander
影响因子：
4.5
作者：
Hamilton, Nicholas
通讯作者：
Hamilton, Nicholas
DOI：
10.1007/978-1-61779-415-5_26
发表时间：
2012-01-01
期刊：
BONE RESEARCH PROTOCOLS, SECOND EDITION
影响因子：
--
作者：
Dallas, Sarah L.;Veno, Patricia A.
通讯作者：
Veno, Patricia A.
影响因子：
6.2
作者：
Bousson, V;Peyrin, F;Laredo, JD
通讯作者：
Laredo, JD