Isolation and Characterization of N-acyl Homoserine Lactone-Producing Bacteria From Cattle Rumen and Swine Intestines.
Isolation and Characterization of N-acyl Homoserine Lactone-Producing Bacteria From Cattle Rumen and Swine Intestines.
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牛瘤胃和猪肠中产生 N-酰基高丝氨酸内酯的细菌的分离和表征
DOI：
10.3389/fcimb.2018.00155
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发表时间：
2018
影响因子：
5.7
通讯作者：
Zhu G
中科院分区：
文献类型：
--
作者：
Yang Y;Zhou M;Hardwidge PR;Cui H;Zhu G
群体感应系统响应细菌种群密度调节基因表达。酰基高丝氨酸内酯是一类存在于牛瘤胃中的群体感应分子，被认为调节肠出血性大肠杆菌的基因表达，从而帮助该病原体在动物胃肠道中存活。然而，在牛和猪胃肠道中产生这些信号分子的特定细菌是未知的。在这里，我们开发了方法来浓缩胃肠液和筛选产生酰基高丝氨酸内酯的细菌。从牛瘤胃中分离到一株铜绿假单胞菌YZ 1，从猪肠道中分离到一株嗜水气单胞菌YZ 2。培养上清液的质谱分析表明，至少有三个特定类别的酰基-高丝氨酸内酯产生的YZ 1，和C4-酰基-高丝氨酸内酯产生的YZ 2。转化大肠大肠杆菌与铜绿假单胞菌或A. Escherichia luxI同源物，可产生短链或长链酰基高丝氨酸内酯。coli合成酰基高丝氨酸内酯的能力，并影响其基因表达、运动性和耐酸性。杆菌这是首次报道从牛瘤胃和猪肠道中分离和鉴定酰基高丝氨酸内酯脱氢酶阳性细菌的研究。
Quorum sensing systems regulate gene expression in response to bacterial population density. Acyl-homoserine lactones are a class of quorum sensing molecules found in cattle rumen that are thought to regulate the gene expression of enterohemorrhagic Escherichia coli and thus help this pathogen survive in animal gastrointestinal tracts. However, the specific bacteria that produce these signaling molecules in bovine and porcine gastrointestinal tracts are unknown. Here we developed methods to concentrate gastrointestinal fluids and screen the bacteria that produce acyl-homoserine lactones. We isolated a Pseudomonas aeruginosa strain YZ1 from cattle rumen, and an Aeromonas hydrophila strain YZ2 from pig intestine. Mass spectrometry analysis of culture supernatants indicated at least three specific classes of acyl-homoserine lactones produced by YZ1, and a C4-acyl-homoserine lactone produced by YZ2. Transformation of E. coli with P. aeruginosa or A. hydrophila luxI homologs,which can produce short- or long-chain acyl-homoserine lactones conferred upon E. coli the ability to synthesize acyl-homoserine lactones and affected gene expression, motility, and acid tolerance of E. coli. This is the first study reporting the isolation and characterization of acyl-homoserine lactone synthase-positive bacteria from cattle rumen and swine intestines.
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影响因子：
3.3
作者：
Mainil, JG;Jacquemin, E;Benz, I
通讯作者：
Benz, I
影响因子：
3.3
作者：
Frydendahl, K
通讯作者：
Frydendahl, K
影响因子：
2.6
作者：
HAVENS, PL;DUNNE, WM;BURD, EM
通讯作者：
BURD, EM
影响因子：
3.6
作者：
Kanamaru, K;Kanamaru, K;Sasakawa, C
通讯作者：
Sasakawa, C
DOI：
10.3390/s131013192
发表时间：
2013-09-30
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Chen JW;Chin S;Tee KK;Yin WF;Choo YM;Chan KG
通讯作者：
Chan KG