The SUMOylation of TAB2 mediated by TRIM60 inhibits MAPK/NF-κB activation and the innate immune response.
The SUMOylation of TAB2 mediated by TRIM60 inhibits MAPK/NF-κB activation and the innate immune response.
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TRIM60 介导的 TAB2 SUMOylation 抑制 MAPK/NF-κB 激活和先天免疫反应
DOI：
10.1038/s41423-020-00564-w
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发表时间：
2021-08
影响因子：
24.1
通讯作者：
Hu H
中科院分区：
文献类型：
--
作者：
Gu Z;Chen X;Yang W;Qi Y;Yu H;Wang X;Gong Y;Chen Q;Zhong B;Dai L;Qi S;Zhang Z;Zhang H;Hu H
TAK1 信号体的激活对于介导针对病原体入侵的先天免疫反应至关重要，并受到多层翻译后修饰的调节，包括泛素化、SUMO 化和磷酸化；然而，潜在的分子机制尚未完全了解。在这项研究中，TRIM60 负向调节 TAK1 信号体的形成和激活。巨噬细胞中 TRIM60 的缺乏会导致 MAPK 和 NF-κB 激活增强，同时促炎细胞因子水平升高，但 IFN-I 水平不会升高。免疫沉淀-质谱分析确定 TAB2 是 TRIM60 进行 SUMO 化而不是泛素化的靶标，导致 TRAF6/TAB2/TAK1 复合物以及下游 MAPK 和 NF-κB 通路的形成受损。 TRIM60介导的TAB2的SUMO化位点被鉴定为K329和K562；用精氨酸取代这些赖氨酸消除了 TAB2 的 SUMOylation。体内实验表明，TRIM60 缺陷小鼠对 LPS 诱导的感染性休克和单核细胞增生李斯特菌感染表现出更高的免疫反应。我们的数据表明，TRIM60 介导的 TAB2 SUMO 化是调节先天免疫反应的新机制，可能为控制抗菌免疫反应的新策略铺平道路。
Activation of the TAK1 signalosome is crucial for mediating the innate immune response to pathogen invasion and is regulated by multiple layers of posttranslational modifications, including ubiquitination, SUMOylation, and phosphorylation; however, the underlying molecular mechanism is not fully understood. In this study, TRIM60 negatively regulated the formation and activation of the TAK1 signalosome. Deficiency of TRIM60 in macrophages led to enhanced MAPK and NF-κB activation, accompanied by elevated levels of proinflammatory cytokines but not IFN-I. Immunoprecipitation-mass spectrometry assays identified TAB2 as the target of TRIM60 for SUMOylation rather than ubiquitination, resulting in impaired formation of the TRAF6/TAB2/TAK1 complex and downstream MAPK and NF-κB pathways. The SUMOylation sites of TAB2 mediated by TRIM60 were identified as K329 and K562; substitution of these lysines with arginines abolished the SUMOylation of TAB2. In vivo experiments showed that TRIM60-deficient mice showed an elevated immune response to LPS-induced septic shock and L. monocytogenes infection. Our data reveal that SUMOylation of TAB2 mediated by TRIM60 is a novel mechanism for regulating the innate immune response, potentially paving the way for a new strategy to control antibacterial immune responses.
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影响因子：
44.1
作者：
Hu H;Sun SC
通讯作者：
Sun SC
DOI：
10.1084/jem.20151426
发表时间：
2016-03-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hu H;Wang H;Xiao Y;Jin J;Chang JH;Zou Q;Xie X;Cheng X;Sun SC
通讯作者：
Sun SC
对 RNA 病毒的先天免疫受 RIG-I 和 MDA5 的暂时且可逆的 sumoylation 调节
DOI：
10.1084/jem.20161015
发表时间：
2017-04-03
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hu MM;Liao CY;Yang Q;Xie XQ;Shu HB
通讯作者：
Shu HB
影响因子：
14.8
作者：
Bak RO;Dever DP;Porteus MH
通讯作者：
Porteus MH
影响因子：
29.7
作者：
Brubaker SW;Bonham KS;Zanoni I;Kagan JC
通讯作者：
Kagan JC