KIFC1 participates in acrosomal biogenesis, with discussion of its importance for the perforatorium in the Chinese mitten crab Eriocheir sinensis
KIFC1 participates in acrosomal biogenesis, with discussion of its importance for the perforatorium in the Chinese mitten crab Eriocheir sinensis
复制标题
KIFC1参与顶体生物发生并探讨其对中华绒螯蟹穿支的重要性
DOI：
10.1007/s00441-009-0800-3
复制
发表时间：
2009-05
影响因子：
3.6
通讯作者：
Ying, Xue-Ping
中科院分区：
文献类型：
--
作者：
Hou, Lin;Yang, Wan-Xi;Zhu, Jun-Quan;Yu, Keming;Ying, Xue-Ping
精子发生是一个复杂的过程，在这个过程中，精原细胞经过增殖和分裂，经过一系列戏剧性的变化，最终产生成熟的精子。许多分子马达参与了这一过程。KIFC1是一种C-末端的动蛋白马达，参与顶体的生物发生和核的形成。我们报道了KIFC1在中华绒螯蟹精子发生过程中的表达谱。KIFc1主要定位于细胞核周围，但也存在于精原细胞和精母细胞的细胞核中。在精子细胞早期，KIFC1开始分布在原顶体小泡所在的核膜上。到精子细胞晚期，顶体上可见KIFC1.免疫细胞化学和超微结构分析表明，KIFC1定位于穿孔器上，穿孔器由顶帽和顶体小管组成。我们证明，在中华绒毛虫精子发生过程中，KIFC1可能在顶体的生物发生及其维持中发挥重要作用。KIFC1也可能是受精过程中顶体外翻所必需的。
Spermatogenesis is a complicated process during which spermatogonia undergo proliferation and divisions leading, after a series of dramatic changes, to the production of mature spermatozoa. Many molecular motors are involved in this process. KIFC1, a C-terminal kinesin motor, participates in acrosome biogenesis and nuclear shaping. We report here the expression profile of KIFC1 during spermatogenesis in the Chinese mitten crab, Eriocheir sinensis. KIFC1 mainly localizes around the nucleus but is also present within the nucleus of the spermatogonium and spermatocyte. At the early spermatid stage, KIFC1 begins to be distributed on the nuclear membrane at the region where the proacrosomal vesicle is located. By the late spermatid stage, KIFC1 is found on the acrosome. Immunocytochemical and ultrastructural analyses have shown that KIFC1 localizes on the perforatorium, which is composed of an apical cap and an acrosomal tubule. We demonstrate that, during spermatogenesis in E. sinensis, KIFC1 probably plays important roles in the biogenesis of the acrosome and in its maintenance. KIFC1 may also be essential for the eversion of the acrosome during fertilization.
登录
查看更多内容
影响因子：
3.6
作者：
K. Toshimori
通讯作者：
K. Toshimori
影响因子：
2.4
作者：
A. S. Benetti;D. C. Santos;M. L. Negreiros-Fransozo;M. A. Scelzo
通讯作者：
A. S. Benetti;D. C. Santos;M. L. Negreiros-Fransozo;M. A. Scelzo
影响因子：
2.7
作者：
Ying Zhang;R. Oko;F. A. van der Hoorn
通讯作者：
Ying Zhang;R. Oko;F. A. van der Hoorn
影响因子：
--
作者：
Wang R;Sperry AO
通讯作者：
Sperry AO
影响因子：
2.5
作者：
Bray, JD;Chennathukuzhi, VM;Hecht, NB
通讯作者：
Hecht, NB