A method for volumetric retinal tissue oxygen tension imaging.
A method for volumetric retinal tissue oxygen tension imaging.
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DOI：
10.1080/02713683.2017.1373823
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发表时间：
2018-01
影响因子：
2
通讯作者：
Shahidi M
中科院分区：
文献类型：
--
作者：
Felder AE;Wanek J;Teng PY;Blair NP;Shahidi M
视网膜氧合不足发生在许多威胁视力的视网膜疾病中，包括糖尿病视网膜病变、视网膜血管闭塞和年龄相关性黄斑变性。因此，评估视网膜氧化的技术对于了解健康和疾病中的视网膜生理学是必要的。本研究的目的是报道一种大鼠视网膜组织氧张力（tPO2）的三维（3D）成像方法。在全身缺氧（N = 6）和缺氧（N = 3）条件下，对Long Evans色素染色大鼠进行成像。在视网膜上水平扫描一条垂直的激光线，获得一系列光学切片的相位延迟磷光图像。从这些图像中，构建每个相位延迟的磷光体积，并生成3D视网膜tPO2体积。通过生成平均tPO2 （MtPO2）和tPO2空间变异（SVtPO2）的视网膜深度曲线，定量分析视网膜tPO2体积。采用混合线性模型测定全身条件（常氧/缺氧）和视网膜深度对MtPO2和SVtPO2的影响。每个三维视网膜tPO2体积约为500 × 750 × 200 μm（水平×垂直×深度），由45张通过视网膜深度的正面tPO2图像组成。绒毛膜视网膜界面MtPO2与全身动脉氧张力显著相关（P = 0.007; N = 9）。全身状况和视网膜深度对MtPO2和svvtpo2均有显著影响，低氧条件下MtPO2和svvtpo2低于常氧条件下，外视网膜高于内视网膜（P < 0.001）。该研究首次证实了视网膜tPO2的3D成像，在视网膜疾病动物模型的生理改变评估中具有潜在的应用前景。
Inadequate retinal oxygenation occurs in many vision-threatening retinal diseases, including diabetic retinopathy, retinal vascular occlusions, and age-related macular degeneration. Therefore, techniques that assess retinal oxygenation are necessary to understand retinal physiology in health and disease. The purpose of the current study is to report a method for the three-dimensional (3D) imaging of retinal tissue oxygen tension (tPO2) in rats. Imaging was performed in Long Evans pigmented rats under systemic normoxia (N = 6) or hypoxia (N = 3). A vertical laser line was horizontally scanned on the retina and a series of optical section phase-delayed phosphorescence images were acquired. From these images, phosphorescence volumes at each phase delay were constructed and a 3D retinal tPO2 volume was generated. Retinal tPO2 volumes were quantitatively analyzed by generating retinal depth profiles of mean tPO2 (MtPO2) and the spatial variation of tPO2 (SVtPO2). The effects of systemic condition (normoxia/hypoxia) and retinal depth on MtPO2 and SVtPO2 were determined by mixed linear model. Each 3D retinal tPO2 volume was approximately 500 × 750 × 200 μm (horizontal × vertical × depth) and consisted of 45 en face tPO2 images through the retinal depth. MtPO2 at the chorioretinal interface was significantly correlated with systemic arterial oxygen tension (P = 0.007; N = 9). There were significant effects of both systemic condition and retinal depth on MtPO2 and SVtPO2, such that both were lower under hypoxia than normoxia and higher in the outer retina than inner retina (P < 0.001). For the first time, 3D imaging of retinal tPO2 was demonstrated, with potential future application for assessment of physiological alterations in animal models of retinal diseases.
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