Ligand-competent fractalkine receptor is expressed on exosomes.
Ligand-competent fractalkine receptor is expressed on exosomes.
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配体能力分裂受体在外泌体上表达。
DOI：
10.1016/j.bbrep.2021.100932
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发表时间：
2021-07
影响因子：
2.7
通讯作者：
Shimaoka M
中科院分区：
文献类型：
--
作者：
Park EJ;Myint PK;Appiah MG;Worawattananutai P;Inprasit J;Prajuabjinda O;Soe ZY;Gaowa A;Kawamoto E;Shimaoka M
据报道，趋化因子受体CX3CR1的表达限于几种细胞类型，包括自然杀伤细胞、细胞毒性T细胞、单核细胞和巨噬细胞。然而，它在外泌体上的表达和功能仍不清楚，外泌体是已知作为细胞间通讯介质的纳米级细胞外囊泡。在这里，我们研究了从各种细胞类型分离的外来体上的CX3CR1表达。虽然我们发现在我们的研究中测试的所有外泌体都高度表达CX3CR1，但这种趋化因子受体仅在其源细胞内表达，而几乎不表达。此外，外泌体CX3CR1能够结合可溶性CX3CL1。因此，我们的研究表明，CX3CR1是一种新的配体能力的外泌体受体。CX3CR1由外来体高度表达。CX3CR1的表达被限制在细胞内，而不是细胞上。外泌体CX3CR1能够结合可溶性CX3CL1。CX3CL1与外来体的结合可能剥夺其来源细胞与该趋化因子结合的机会。
Expression of chemokine receptor CX3CR1 is reportedly restricted to several cell types including natural killer cells, cytotoxic T cells, monocytes, and macrophages. However, its expression and function on exosomes, which are nanosized extracellular vesicles known to act as mediators of intercellular communications, remain unclear. Here, we investigated CX3CR1 expression on exosomes isolated from various cell types. Although we found that all the exosomes tested in our study highly expressed CX3CR1, this chemokine receptor was expressed only inside, but barely on, their source cells. Moreover, exosomal CX3CR1 was capable of binding soluble CX3CL1. Therefore, our study suggests that CX3CR1 is a novel and ligand-competent exosome receptor. CX3CR1 is highly expressed by exosomes. Expression of CX3CR1 is restricted within, but not on, the cells. Exosomal CX3CR1 is capable of binding soluble CX3CL1. CX3CL1 binding of exosomes may deprive their source cells of the chance to bind this chemokine.
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DOI：
10.1007/978-3-030-36667-4_1
发表时间：
2020-01-01
期刊：
TUMOR MICROENVIRONMENT: THE ROLE OF CHEMOKINES, PT A
影响因子：
--
作者：
Conroy, Melissa J.;Lysaght, Joanne
通讯作者：
Lysaght, Joanne
影响因子：
3.2
作者：
Sung, Bong Hwan;Weaver, Alissa M.
通讯作者：
Weaver, Alissa M.
影响因子：
7.5
作者：
Marchese, Adriano
通讯作者：
Marchese, Adriano
影响因子：
5.4
作者：
Bain, Calum C.;Mowat, Allan McI
通讯作者：
Mowat, Allan McI
间充质干细胞释放的 CCR2 阳性外泌体抑制巨噬细胞功能并减轻缺血/再灌注引起的肾损伤。
影响因子：
4.3
作者：
Shen B;Liu J;Zhang F;Wang Y;Qin Y;Zhou Z;Qiu J;Fan Y
通讯作者：
Fan Y