Small RNA sequencing reveals a novel tsRNA-06018 playing an important role during adipogenic differentiation of hMSCs.
Small RNA sequencing reveals a novel tsRNA-06018 playing an important role during adipogenic differentiation of hMSCs.
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小RNA测序揭示了一种新型tsRNA-06018在hMSCs的脂肪形成分化过程中发挥重要作用
DOI：
10.1111/jcmm.15858
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发表时间：
2020-11
影响因子：
5.3
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Wang T;Cao L;He S;Long K;Wang X;Yu H;Ma B;Xu X;Li W
转移RNA的小RNA（TSRNA）是一种新型的非编码RNA衍生物，能够调节这些TSRNA在调节人骨骨髓间充质干细胞（HMSC）的脂肪质分化中，这些TSRNA在调节人类骨骨髓中的作用是什么作用。转录组测序，导致我们识别TSRNA-06018是基于TSRNA的表达曲线的目标，当TsRNA-06018被击倒。 STC2的3'-无翻译区（3'-UTR）另外，我们确定击倒TSRNA -06018和过表达的STC2增加了细胞外信号调节的激酶1/2（ERK1/2）细胞内的磷酸化，我们还评估了HMSC的脂肪生成量与TSRNA -06018的同种。单独使用小RNA测序我们在掺杂过程中对HMSC中的TSRNA进行了介绍，这使我们识别TsRNA -06018是这种分化过程的新调节剂。
Transfer RNA‐derived small RNAs (tsRNAs), a novel type of non‐coding RNA derivative, are able to regulate a wide range of biological processes. What role these tsRNAs play in the regulation of human bone marrow mesenchymal stem cell (hMSCs) adipogenic differentiation remains uncertain. We induced the adipogenic differentiation of human bone marrow mesenchymal cells (hMSCs) and then performed small RNA transcriptomic sequencing, leading us to identify tsRNA‐06018 as a target of interest based upon resultant the tsRNA expression profiles. When tsRNA‐06018 was knocked down, this led to the inhibition of adipogenesis and a decrease in adipogenic marker expression. When STC2 was overexpressed, this impaired the adipogenic differentiation of these cells. We further used luciferase reporter assays to confirm that tsRNA‐06018 directly binds the 3′‐untranslated region (3′‐UTR) of STC2. In addition, we determined that both knocking down tsRNA‐06018 and overexpressing STC2 increased extracellular signal‐regulated kinase 1/2 (ERK1/2) phosphorylation within cells. We also assessed that the adipogenic differentiation of hMSCs in which tsRNA‐06018 was knocked down was further enhanced upon the addition of the ERK1/2 inhibitor U0126 as compared tsRNA‐06018 knockdown alone. Taken together, using small RNA sequencing we profiled tsRNAs in hMSCs during the process of adipogenesis, leading us to identify tsRNA‐06018 as a novel regulator of this differentiation process. This tsRNA was able to regulate adipogenic differentiation by targeting STC2 via the ERK1/2 signalling pathway.
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影响因子：
64.5
作者：
Goodarzi H;Liu X;Nguyen HC;Zhang S;Fish L;Tavazoie SF
通讯作者：
Tavazoie SF
影响因子：
12.3
作者：
Galasso M;Sana ME;Volinia S
通讯作者：
Volinia S
DOI：
10.1146/annurev.pathol.4.110807.092222
发表时间：
2009
期刊：
Annual review of pathology
影响因子：
--
作者：
Lee YS;Dutta A
通讯作者：
Dutta A
影响因子：
5.3
作者：
López JJ;Jardín I;Cantonero Chamorro C;Duran ML;Tarancón Rubio MJ;Reyes Panadero M;Jiménez F;Montero R;González MJ;Martínez M;Hernández MJ;Brull JM;Corbacho AJ;Delgado E;Granados MP;Gómez-Gordo L;Rosado JA;Redondo PC
通讯作者：
Redondo PC
影响因子：
4.5
作者：
Dooley, Christopher M.;Wali, Neha;Busch-Nentwich, Elisabeth M.
通讯作者：
Busch-Nentwich, Elisabeth M.