Weight-independent changes in blood glucose homeostasis after gastric bypass or vertical sleeve gastrectomy in rats.
Weight-independent changes in blood glucose homeostasis after gastric bypass or vertical sleeve gastrectomy in rats.
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DOI：
10.1053/j.gastro.2011.05.050
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发表时间：
2011-09
期刊：
影响因子：
29.4
通讯作者：
Sandoval DA
中科院分区：
文献类型：
--
作者：
Chambers AP;Jessen L;Ryan KK;Sisley S;Wilson-Pérez HE;Stefater MA;Gaitonde SG;Sorrell JE;Toure M;Berger J;D'Alessio DA;Woods SC;Seeley RJ;Sandoval DA
Roux-en-Y胃分流术(RYGB)和垂直袖状胃切除术(VSG)减轻肥胖患者的体重并改善糖代谢，尽管目前尚不清楚代谢变化是否独立于体重减轻。我们研究了RYGB或VSG后大鼠糖代谢的变化。将接受RYGB或VSG的大鼠与随机喂养或与接受RYGB的动物配对喂养的假手术大鼠进行比较。进行腹膜糖耐量和胰岛素敏感性试验以评估血糖功能，而不依赖于胰岛素的反应。高胰岛素正血糖钳夹被用来比较每一次手术后特定组织胰岛素敏感性的变化。采用混合餐耐量试验评估每次手术对餐后GLP-1(7-36)释放和糖耐量的影响，并对给予GLP-1受体拮抗剂exendin9-39的大鼠进行检测。服用RYGB或VSG后，糖耐量和胰岛素敏感性改善，与体重减轻成比例。与随机喂养或配对喂养的大鼠相比，服用RYGB或VSG的大鼠肝脏对胰岛素的敏感性显著提高，而所有组的葡萄糖清除情况相似。在混餐耐力试验中，服用RYGB和VSG的大鼠血浆GLP-1(7-36)和胰岛素水平显著高于配对喂养或随机喂养的大鼠，且差异有统计学意义。给予GLP-1受体拮抗剂可阻止服用RYGB或VSG的大鼠餐后血糖和胰岛素反应的改善。在肥胖大鼠中，VSG在增加GLP-1的分泌和胰岛素的分泌以及改善肝脏对胰岛素的敏感性方面与RYGB一样有效；这些效应与体重减轻无关。
Roux-en-Y gastric bypass (RYGB) and vertical sleeve gastrectomy (VSG) reduce weight and improve glucose metabolism in obese patients, although it is not clear if metabolic changes are independent of weight loss. We investigated alterations in glucose metabolism in rats following RYGB or VSG. Rats underwent RYGB or VSG and were compared to sham-operated rats that were fed ad lib or pair-fed with animals that received RYGB. Intraperitoneal glucose tolerance and insulin sensitivity tests were performed to assess glycemic function, independent of the incretin response. A hyperinsulinemic euglycemic clamp was used to compare tissue-specific changes in insulin sensitivity following each procedure. A mixed-meal tolerance tests was used to assess the effect of each surgery on post-prandial release of GLP-1(7–36) and glucose tolerance, and were also performed in rats given the GLP-1 receptor antagonist exendin9–39. Following RYGB or VSG, glucose tolerance and insulin sensitivity improved, in proportion to weight loss. Hepatic insulin sensitivity was significantly better in rats that received RYGB or VSG, compared with rats fed ad lib or pair-fed, whereas glucose clearance was similar in all groups. During the mixed-meal tolerance test, plasma levels of GLP-1(7–36) and insulin were greatly and comparably increased in rats that received RYGB and VSG, compared with those that were pair-fed or fed ad lib. Administration of a GLP-1 receptor antagonist prevented improvements in glucose and insulin responses after a meal among rats that received RYGB or VSG. In obese rats, VSG is as effective as RYGB at increasing secretion of GLP-1 and insulin secretion and at improving hepatic sensitivity to insulin; these effects are independent of weight loss.
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仅在发生大量体重减轻并与减肥幅度相关后，才能观察到胃旁路手术后的外周葡萄糖吸收的改善。
影响因子：
3.2
作者：
Campos, Guilherme M.;Rabl, Charlotte;Peeva, Sofia;Ciovica, Ruxandra;Rao, Madhu;Schwarz, Jean-Marc;Havel, Peter;Schambelan, Morris;Mulligan, Kathleen
通讯作者：
Mulligan, Kathleen
影响因子：
29.4
作者：
Stefater MA;Pérez-Tilve D;Chambers AP;Wilson-Pérez HE;Sandoval DA;Berger J;Toure M;Tschöp M;Woods SC;Seeley RJ
通讯作者：
Seeley RJ
弧形胰高血糖素样肽1受体调节葡萄糖稳态，但不调节食物摄入量。
影响因子：
7.7
作者：
Sandoval, Darleen A.;Bagnol, Didier;Woods, Stephen C.;D'Alessio, David A.;Seeley, Randy J.
通讯作者：
Seeley, Randy J.
影响因子：
2.9
作者：
Rizzello, Mario;Abbatini, Francesca;Basso, Nicola
通讯作者：
Basso, Nicola
影响因子：
5.8
作者：
Laferrere, Blandine;Teixeira, Julio;Olivan, Blanca
通讯作者：
Olivan, Blanca