Anaerobic storage of red blood cells in a novel additive solution improves in vivo recovery.
Anaerobic storage of red blood cells in a novel additive solution improves in vivo recovery.
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DOI：
10.1111/j.1537-2995.2008.02038.x
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发表时间：
2009-03
期刊：
影响因子：
2.9
通讯作者：
AuBuchon JP
中科院分区：
文献类型：
--
作者：
Dumont LJ;Yoshida T;AuBuchon JP
在初步研究中，厌氧RBC储存减少了氧化损伤和磷脂酰丝氨酸暴露，同时保持ATP水平。本研究的目的是比较在厌氧环境中使用OFAS 3添加剂溶液储存6周和9周的自体RBC与在AS-3中有氧储存6周的对照RBC的24小时回收率和寿命。8例受试者入组一项随机、交叉研究。从每例受试者中采集两次全血，在≥12周时分离至CP 2D和白细胞减少。对照品储存在AS-3中。OFAS 3中的测试单元用氩气耗氧，然后在1-6°C的厌氧室中储存9周。在每个储存期结束时，用51 Cr和99 mTc标记RBC，并按照标准方法重新输注至受试者体内，以确定双标记回收率和寿命。使用配对重复测量ANOVA进行假设检验。第6周时，厌氧储存试验RBC的回收率显著优于对照组（p=0.023）。第9周的试验单位与第6周的对照单位无差异（p=0.73）。RBC特征的其他体外测量遵循相同的趋势。两个测试单元在9周时具有> 1%的溶血。与对照相比，OFAS-3中厌氧储存的RBC在6周时具有上级回收率，在9周时具有等同的回收率，寿命无变化。RBC的厌氧储存可以在储存6周时提供用于输血的改善的RBC，并且可以使储存延长超过当前的42天限制。
In preliminary studies, anaerobic RBC storage reduced oxidative damage and phosphatidylserine exposure while maintaining ATP levels. The purpose of this study was to compare the 24-hour recovery and lifespan of autologous RBCs stored 6 and 9 weeks using OFAS3 additive solution in an anaerobic environment, compared to control RBCs aerobically stored in AS-3 for 6 weeks. Eight subjects were entered into a randomized, cross-over study. Whole blood was collected from each subject twice separated by ≥12 wk into CP2D and leukocyte reduced. Controls were stored in AS-3. Test units in OFAS3 were oxygen depleted with Argon then stored 9 wk in an anaerobic chamber at 1–6°C. At the end of each storage period, RBC were labeled with 51Cr and 99mTc and reinfused to the subject following standard methods to determine double label recovery and lifespan. Hypotheses tests were conducted using paired, repeated-measures ANOVA. Recovery for the anaerobically stored test RBC was significantly better than control at 6 weeks (p=0.023). Test units at 9 wk were not different than the 6 wk control units (p=0.73). Other in vitro measures of RBC characteristics followed the same trend. Two test units at 9 wk had hemolysis > 1%. Anaerobically stored RBC in OFAS-3 has superior recovery at 6 weeks compared to the Controls and equivalent recovery at 9 weeks with no change in lifespan. Anaerobic storage of RBC may provide an improved RBC for transfusion at 6 wk of storage and may enable extending storage beyond the current 42 day limit.
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