The Dynamic Immune Response of Yellow Catfish (Pelteobagrus fulvidraco) Infected With Edwardsiella ictaluri Presenting the Inflammation Process.
The Dynamic Immune Response of Yellow Catfish (Pelteobagrus fulvidraco) Infected With Edwardsiella ictaluri Presenting the Inflammation Process.
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黄颡鱼感染鲶鱼爱德华氏菌后的动态免疫反应呈现炎症过程
DOI：
10.3389/fimmu.2021.625928
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发表时间：
2021
影响因子：
7.3
通讯作者：
Wei KJ
中科院分区：
文献类型：
--
作者：
Zhou X;Zhang GR;Ji W;Shi ZC;Ma XF;Luo ZL;Wei KJ
黄颡鱼爱德华氏菌是养殖黄颡鱼的一种极具破坏性的病原菌，因此研究黄颡鱼对细菌感染的免疫应答是十分必要的。本研究利用RNA-Seq技术研究了黄颡鱼感染大肠杆菌后8个不同时间点（h）两种不同组织的免疫应答。蜱感染。在感染后3 h和6 h，脾脏和肝脏中差异表达基因（DEG）的数量分别较低。随后，脾脏中DEGs的数量在72 h时达到最大值，而肝脏中DEGs的数量在24 ~ 120 h维持在较高水平。不同时间点DEG的GO和KEGG富集分析发现，细胞因子在6 ~ 120 h持续转录;而肝脏是分泌补体系统组分的主要器官，代谢调节在12 ~ 120 h被激活。此外，还对黄颡鱼的炎症反应进行了综述，包括模式识别受体、炎症细胞因子、趋化因子、补体以及炎症相关信号通路。通过qRT-PCR检测的9个基因的相似表达趋势验证了转录组分析的准确性。从脾脏和肝脏中获得的不同转录组谱将有助于更好地了解鱼类对细菌感染的动态免疫反应，并将为制定有效的预防和控制鱼类疾病的措施提供基础信息。
Edwardsiella ictaluri is a highly destructive pathogen in cultured yellow catfish, thus it was very necessary to study the immune response of yellow catfish against bacterial infection. In this study, RNA-Seq technology was used to study the immune response in two distinct tissues of yellow catfish at eight different time points (h) after E. ictaluri infection. The number of differentially expressed genes (DEGs) in the spleen and liver was low at 3 h and 6 h post-infection, respectively. Afterwards, the most number of DEGs in the spleen was detected at 72 h, while the number of DEGs in the liver maintained a high level from 24 h to 120 h. The GO and KEGG enrichment analyses of DEGs at different time points uncovered that cytokines were continuously transcribed at 6 h to 120 h; whereas the liver is the main organ that secretes the components of the complement system, and metabolic regulation was activated from 12 h to 120 h. Moreover, an overview of the inflammation response of yellow catfish was exhibited including pattern-recognition receptors, inflammatory cytokines, chemokines, complements, and inflammation-related signal pathways. The similar expression tendency of nine genes by qRT-PCR validated the accuracy of transcriptome analyses. The different transcriptomic profiles obtained from the spleen and liver will help to better understand the dynamic immune response of fish against bacterial infection, and will provide basic information for establishing effective measures to prevent and control diseases in fish.
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影响因子：
8.7
作者：
Dinarello CA
通讯作者：
Dinarello CA
影响因子：
2.9
作者：
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Dang Y;Xu X;Shen Y;Hu M;Zhang M;Li L;Lv L;Li J
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Li J
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作者：
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影响因子：
2.9
作者：
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通讯作者：
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