Solid-supported microorganism of Burkholderia cenocepacia cultured via solid state fermentation for biodiesel production: Optimization and kinetics
Solid-supported microorganism of Burkholderia cenocepacia cultured via solid state fermentation for biodiesel production: Optimization and kinetics
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通过固态发酵培养的新洋葱伯克霍尔德菌固体支持微生物用于生物柴油生产：优化和动力学
DOI：
10.1016/j.apenergy.2013.08.009
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发表时间：
2014
期刊：
影响因子：
11.2
通讯作者：
Chen Weiyi
中科院分区：
文献类型：
--
作者：
Liu Yun;Li Chong;Wang Shihui;Chen Weiyi
研究了新洋葱伯克霍尔德氏菌（Burkholderia cenocepacia）固体发酵生产生物柴油的工艺条件和动力学。采用Plackett-Burman设计和Box-Behnken法对影响生物柴油产率的操作参数进行了优化。的生物柴油产量ca。在醇油摩尔比为4.3：1、固载菌量为1.63 g/mL、加水量为4.6%（wt/wt）、温度为44.2° C的优化条件下，产率为91.3%。在此基础上，建立了具有竞争性醇抑制的乒乓-比比机理动力学模型。该模型的相关系数为97.60%，动力学参数为vmax = 3.88mmol/（Lmin），Km TG= 0.27mmol/L，Km C2 H5 OH= 4.03mmol/L，Ki = 0.34mmol/L。此外，还计算了该生物催化剂的活化能（Ea）为37.95kJ/mol。固体微生物乙醇分解反应的限速步骤是甘油二酯转化为甘油一酯。最后，在连续的批式反应中评价了固载微生物的可重复使用性，在288 h重复使用后，显示出原始活性的66.9%。
The objectives of this work were to investigate the optimization and kinetics of ethanolysis for biodiesel production by solid-supported microorganism of Burkholderia cenocepacia cultured via solid state fermentation (SSF). Operational parameters affecting the biodiesel yield were successively optimized by Plackett–Burman design followed with Box–Behnken method. The biodiesel yield of ca. 91.3% was verified under the optimized conditions of 4.3: 1 of alcohol/oil molar ratio, 1.63 g/mL of solid-supported microorganism mass, 4.6%(wt/wt) of water and 44.2° C of temperature. Furthermore, the kinetic model was developed on the basis of Ping Pong Bi Bi mechanism with competitive alcohol inhibition. The proposed model had a satisfactory correlation coefficient of R 2= 97.60%, and kinetic parameters of v max= 3.88 mmol/(L min), K m TG= 0.27 mmol/L, K m C 2 H 5 OH= 4.03 mmol/L, and K i= 0.34 mmol/L were obtained. In addition, the calculated activation energy (E a) of the biocatalyst was estimated to be 37.95 kJ/mol. The rate-limiting reaction step for the ethanolysis by solid-supported microorganism in tert-butanol was demonstrated to be the conversion of di-glyceride to mono-glyceride. Finally, the reusability of the solid-supported microorganism was evaluated in successive batch reactions and presented 66.9% of original activity after 288 h repeated usage.
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DOI：
10.1016/j.fuproc.2010.12.023
发表时间：
2011-05
期刊：
Fuel and Energy Abstracts
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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