Fine mapping of powdery mildew and stripe rust resistance genes Pm5V/Yr5V transferred from Dasypyrum villosum into wheat without yield penalty
Fine mapping of powdery mildew and stripe rust resistance genes Pm5V/Yr5V transferred from Dasypyrum villosum into wheat without yield penalty
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白粉病和条锈病抗性基因 Pm5V/Yr5V 从 Dasypyrum villusum 转移到小麦中的精细定位，且没有产量损失
DOI：
10.1007/s00122-022-04206-9
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发表时间：
2022-08
期刊：
影响因子：
--
通讯作者：
Aizhong Cao
中科院分区：
文献类型：
--
作者：
Ruiqi Zhang;Chuntian Lu;Xiangru Meng;Yali Fan;Jie Du;Runran Liu;Yigao Feng;Liping Xing;Petr Cápal;Kateřina Holušová;Jaroslav Doležel;Yiwei Wang;Huanqing Mu;Bingxiao Sun;Fu Hou;Ruonan Yao;Chuanxi Xiong;Yang Wang;Peidu Chen;Aizhong Cao
将小麦抗白粉病和抗条锈病新基因Pm5V/Yr5V从簇毛麦(Dasypyrum Villosum)中导入，并在5VS上进行了精细定位，研究了它们对产量相关性状的影响。小麦白粉病和条锈病严重威胁着世界小麦生产。簇毛麦(Dasypyrum villosum，2n=2x=14，Vv)是小麦的近缘种，是小麦遗传改良的重要抗病基因资源。在这里，我们描述了一个平台，用于将来自毛白粉菌的抗性基因快速导入到小麦D基因组中。一套完整的新小麦D。建立了V(D)二体代换系和11个D/V罗伯逊易位系，并用分子细胞遗传学方法对它们进行了鉴定。T5D1.5V5S新品系NAU1908同时表现对白粉病和条锈病的抗性，与5VS相关的抗性分别由苗期抗性基因Pm5V和成株抗性基因Yr5V决定。我们对染色体臂5VS进行流动排序，并使用Illumina NovaSeq 6000系统对其进行测序，该系统允许我们为Pm5V/Yr5V的遗传图谱生成5VS特异性标记。精细定位表明，Pm5V和Yr5V紧密连锁，参照中国春5DS的序列，定位范围缩小到约0.9Mb。在该区域，与TraesCS5D02G044300同源的5VS支架24,874上的一个NLR基因是Pm5V最有可能的候选基因。软粒和硬粒T5DL.5V5S导入在不同的小麦遗传背景下都能同时抵抗白粉病和条锈病，而不会影响产量。与NIL-5DL·5DS相比，NIL-5DL·5V5S株高显著降低，产量显著增加。这些结果表明，Pm5V/Yr5V品系可能在小麦抗病育种中具有潜在的应用价值。
The novel wheat powdery mildew and stripe rust resistance genes Pm5V/Yr5V are introgressed from Dasypyrum villosum and fine mapped to a narrowed region in 5VS, and their effects on yield-related traits were characterized. The powdery mildew and stripe rust seriously threaten wheat production worldwide. Dasypyrum villosum (2n = 2x = 14, VV), a relative of wheat, is a valuable resource of resistance genes for wheat improvement. Here, we describe a platform for rapid introgression of the resistance genes from D. villosum into the wheat D genome. A complete set of new wheat-D. villosum V (D) disomic substitution lines and 11 D/V Robertsonian translocation lines are developed and characterized by molecular cytogenetic method. A new T5DL·5V5S line NAU1908 shows resistance to both powdery mildew and stripe rust, and the resistances associated with 5VS are confirmed to be conferred by seedling resistance gene Pm5V and adult-plant resistance gene Yr5V, respectively. We flow-sort chromosome arm 5VS and sequence it using the Illumina NovaSeq 6000 system that allows us to generate 5VS-specific markers for genetic mapping of Pm5V/Yr5V. Fine mapping shows that Pm5V and Yr5V are closely linked and the location is narrowed to an approximately 0.9 Mb region referencing the sequence of Chinese Spring 5DS. In this region, a NLR gene in scaffold 24,874 of 5VS orthologous to TraesCS5D02G044300 is the most likely candidate gene for Pm5V. Soft- and hard-grained T5DL·5V5S introgressions confer resistance to both powdery mildew and stripe rust in diverse wheat genetic backgrounds without yield penalty. Meanwhile, significant decrease in plant height and increase in yield were observed in NIL-5DL·5V5S compared with that in NIL-5DL·5DS. These results indicate that Pm5V/Yr5V lines might have the potential value to facilitate wheat breeding for disease resistance.
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影响因子：
46.9
作者：
Gaurav K;Arora S;Silva P;Sánchez-Martín J;Horsnell R;Gao L;Brar GS;Widrig V;John Raupp W;Singh N;Wu S;Kale SM;Chinoy C;Nicholson P;Quiroz-Chávez J;Simmonds J;Hayta S;Smedley MA;Harwood W;Pearce S;Gilbert D;Kangara N;Gardener C;Forner-Martínez M;Liu J;Yu G;Boden SA;Pascucci A;Ghosh S;Hafeez AN;O'Hara T;Waites J;Cheema J;Steuernagel B;Patpour M;Justesen AF;Liu S;Rudd JC;Avni R;Sharon A;Steiner B;Kirana RP;Buerstmayr H;Mehrabi AA;Nasyrova FY;Chayut N;Matny O;Steffenson BJ;Sandhu N;Chhuneja P;Lagudah E;Elkot AF;Tyrrell S;Bian X;Davey RP;Simonsen M;Schauser L;Tiwari VK;Randy Kutcher H;Hucl P;Li A;Liu DC;Mao L;Xu S;Brown-Guedira G;Faris J;Dvorak J;Luo MC;Krasileva K;Lux T;Artmeier S;Mayer KFX;Uauy C;Mascher M;Bentley AR;Keller B;Poland J;Wulff BBH
通讯作者：
Wulff BBH
影响因子：
30.8
作者：
Li G;Wang L;Yang J;He H;Jin H;Li X;Ren T;Ren Z;Li F;Han X;Zhao X;Dong L;Li Y;Song Z;Yan Z;Zheng N;Shi C;Wang Z;Yang S;Xiong Z;Zhang M;Sun G;Zheng X;Gou M;Ji C;Du J;Zheng H;Doležel J;Deng XW;Stein N;Yang Q;Zhang K;Wang D
通讯作者：
Wang D
影响因子：
1.9
作者：
Gradzielewska, Agnieszka
通讯作者：
Gradzielewska, Agnieszka
影响因子：
56.9
作者：
Periyannan, Sambasivam;Moore, John;Lagudah, Evans
通讯作者：
Lagudah, Evans
影响因子：
7.2
作者：
Hurni, Severine;Brunner, Susanne;Keller, Beat
通讯作者：
Keller, Beat